Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7336 | 3' | -54.4 | NC_001900.1 | + | 755 | 0.68 | 0.644335 |
Target: 5'- ----cGCUgGUGCGGCGCguACuGGCa -3' miRNA: 3'- gaacaCGAgCAUGCCGUGguUG-CCGg -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 834 | 0.69 | 0.534139 |
Target: 5'- cCUUGcgacUGC-CaGUACGcgccGCACCAGCGGCUg -3' miRNA: 3'- -GAAC----ACGaG-CAUGC----CGUGGUUGCCGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 1237 | 0.67 | 0.69959 |
Target: 5'- -gUGUaCUCGgugGCGGCGgCAGCGGa- -3' miRNA: 3'- gaACAcGAGCa--UGCCGUgGUUGCCgg -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 1437 | 0.66 | 0.753162 |
Target: 5'- --aGUGCUgGUcgcggGCGGCgaccGCCAGCGcguugaacGCCg -3' miRNA: 3'- gaaCACGAgCA-----UGCCG----UGGUUGC--------CGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 1614 | 0.68 | 0.644335 |
Target: 5'- --gGUGCuUCGUGCGGUggACCAccuGC-GCCu -3' miRNA: 3'- gaaCACG-AGCAUGCCG--UGGU---UGcCGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 4992 | 0.71 | 0.461015 |
Target: 5'- ---aUGCUCGUcgggcuccagcACGGCGgCGGgGGCCg -3' miRNA: 3'- gaacACGAGCA-----------UGCCGUgGUUgCCGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 6636 | 0.66 | 0.732029 |
Target: 5'- ---cUGCgUGUGCGGguCgAACGGCUg -3' miRNA: 3'- gaacACGaGCAUGCCguGgUUGCCGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 7928 | 0.66 | 0.710485 |
Target: 5'- --cGUGUucuUCGUcUGGUccugGCUGACGGCCg -3' miRNA: 3'- gaaCACG---AGCAuGCCG----UGGUUGCCGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 10265 | 0.66 | 0.753162 |
Target: 5'- uCUUGaucaggcgGCUCucUGCGGCcCUGAUGGCCu -3' miRNA: 3'- -GAACa-------CGAGc-AUGCCGuGGUUGCCGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 16987 | 0.7 | 0.471152 |
Target: 5'- -gUGUGgUCGcacuCGGCAUgGACGGCa -3' miRNA: 3'- gaACACgAGCau--GCCGUGgUUGCCGg -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 17411 | 0.68 | 0.633206 |
Target: 5'- --cGUGUUCG-ACGGUGCCAugcaAgGGCUu -3' miRNA: 3'- gaaCACGAGCaUGCCGUGGU----UgCCGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 21576 | 0.66 | 0.742653 |
Target: 5'- uCUUGUGgaUcUG-GGCugCGAUGGCCg -3' miRNA: 3'- -GAACACgaGcAUgCCGugGUUGCCGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 23237 | 0.69 | 0.566754 |
Target: 5'- ---cUGCUCGgcCGGCucaaCGACGGUCu -3' miRNA: 3'- gaacACGAGCauGCCGug--GUUGCCGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 23500 | 0.69 | 0.530913 |
Target: 5'- ----cGCUCGUcguCGGCACCAuccccggugaggcuGCaGGCCu -3' miRNA: 3'- gaacaCGAGCAu--GCCGUGGU--------------UG-CCGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 25653 | 0.66 | 0.72775 |
Target: 5'- -aUGUGCUCGcggaucaUcgccgcgacgugagGCGGCACgGucacCGGCCg -3' miRNA: 3'- gaACACGAGC-------A--------------UGCCGUGgUu---GCCGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 27567 | 0.67 | 0.688627 |
Target: 5'- --gGUGCgcCGcUACauCGCCGACGGCCg -3' miRNA: 3'- gaaCACGa-GC-AUGccGUGGUUGCCGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 28055 | 0.68 | 0.610956 |
Target: 5'- --cGUGCccgcCGgggGCGGCGUCAACGuGCCg -3' miRNA: 3'- gaaCACGa---GCa--UGCCGUGGUUGC-CGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 28201 | 0.69 | 0.544943 |
Target: 5'- cCUUGUGCgaugUCGU-UGGCcucguccuuGCgGACGGCCu -3' miRNA: 3'- -GAACACG----AGCAuGCCG---------UGgUUGCCGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 30460 | 1.13 | 0.000624 |
Target: 5'- uCUUGUGCUCGUACGGCACCAACGGCCg -3' miRNA: 3'- -GAACACGAGCAUGCCGUGGUUGCCGG- -5' |
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7336 | 3' | -54.4 | NC_001900.1 | + | 31691 | 0.67 | 0.666547 |
Target: 5'- gCUUG-GCUCcgguCGGC-CCGAacCGGCCg -3' miRNA: 3'- -GAACaCGAGcau-GCCGuGGUU--GCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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