Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7337 | 3' | -54.1 | NC_001900.1 | + | 36757 | 0.66 | 0.771993 |
Target: 5'- cGGCGGAUcaGCUcCAgCG-AGGUCCGUg -3' miRNA: 3'- -CUGCUUG--CGAaGUgGCuUCCAGGCGg -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 11864 | 0.66 | 0.782081 |
Target: 5'- gGGCGAggACGCcgacucgaUCGCCGA--GUCCGCg -3' miRNA: 3'- -CUGCU--UGCGa-------AGUGGCUucCAGGCGg -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 17169 | 0.66 | 0.75139 |
Target: 5'- -gUGAACaucgccaaggGCUUCACCGAcguggugucgcaGGGUCCGg- -3' miRNA: 3'- cuGCUUG----------CGAAGUGGCU------------UCCAGGCgg -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 21560 | 0.66 | 0.740898 |
Target: 5'- uGCGAugGCcgcgaaCGCCGAgaGGGUgaaCGCCu -3' miRNA: 3'- cUGCUugCGaa----GUGGCU--UCCAg--GCGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 1398 | 0.66 | 0.771993 |
Target: 5'- -cCGcACGCUUCugCGcGGGGUCCaucggGCUg -3' miRNA: 3'- cuGCuUGCGAAGugGC-UUCCAGG-----CGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 4235 | 0.66 | 0.757627 |
Target: 5'- aGACG-ACGCgaguuguuuggggUCACCGccGG-CCGCUu -3' miRNA: 3'- -CUGCuUGCGa------------AGUGGCuuCCaGGCGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 13818 | 0.67 | 0.719591 |
Target: 5'- -gUGGACGCaguagCagACCGGAGGaUCCGCUu -3' miRNA: 3'- cuGCUUGCGaa---G--UGGCUUCC-AGGCGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 29840 | 0.67 | 0.697932 |
Target: 5'- aGGC-AGCGCgagcugUCAUCagguGGAGGUCCGCa -3' miRNA: 3'- -CUGcUUGCGa-----AGUGG----CUUCCAGGCGg -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 5020 | 0.67 | 0.708799 |
Target: 5'- -cCGcAACGCcUC-CCGGAGGgagUCGCCu -3' miRNA: 3'- cuGC-UUGCGaAGuGGCUUCCa--GGCGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 43572 | 0.67 | 0.702287 |
Target: 5'- cGAUGGACGCggagcaggcggcgCACCGAGcuGGcCgGCCg -3' miRNA: 3'- -CUGCUUGCGaa-----------GUGGCUU--CCaGgCGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 37631 | 0.68 | 0.639519 |
Target: 5'- cGACGGuuucaagcucgaccACGCcaagaUCuCCGAGGGUCCGUUc -3' miRNA: 3'- -CUGCU--------------UGCGa----AGuGGCUUCCAGGCGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 17935 | 0.68 | 0.676013 |
Target: 5'- -cCGAACGCUUCcCCGAucuggggcAGGUacggGCCg -3' miRNA: 3'- cuGCUUGCGAAGuGGCU--------UCCAgg--CGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 17497 | 0.68 | 0.676013 |
Target: 5'- aGGCGAugGg-UCAgcUCGguGGUCCGCUg -3' miRNA: 3'- -CUGCUugCgaAGU--GGCuuCCAGGCGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 43308 | 0.68 | 0.653925 |
Target: 5'- aGACGuugcucACGCUggcucccucacgUCggGCCGAccguGGGUCUGCCa -3' miRNA: 3'- -CUGCu-----UGCGA------------AG--UGGCU----UCCAGGCGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 35151 | 0.68 | 0.664985 |
Target: 5'- cGACGAcuACGCUgaccUgGCCGAggcGGGUCaguugucgaGCCg -3' miRNA: 3'- -CUGCU--UGCGA----AgUGGCU---UCCAGg--------CGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 31725 | 0.69 | 0.576505 |
Target: 5'- aGGCGGAC-CUUCGgUGAAGcGUUCGUCa -3' miRNA: 3'- -CUGCUUGcGAAGUgGCUUC-CAGGCGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 18149 | 0.7 | 0.511802 |
Target: 5'- uGCGucgGCGCagUCGCCGAguGGGUCgGCUc -3' miRNA: 3'- cUGCu--UGCGa-AGUGGCU--UCCAGgCGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 3023 | 0.71 | 0.490882 |
Target: 5'- -cCGAAgGCg--ACCGGcgacGGGUCCGCCu -3' miRNA: 3'- cuGCUUgCGaagUGGCU----UCCAGGCGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 22511 | 0.71 | 0.490882 |
Target: 5'- cGCGAACGCaUCGCUGGugacgaaaagAGGUgCGCUu -3' miRNA: 3'- cUGCUUGCGaAGUGGCU----------UCCAgGCGG- -5' |
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7337 | 3' | -54.1 | NC_001900.1 | + | 1702 | 0.71 | 0.450299 |
Target: 5'- --aGAGCGCgcUgACCGggGGUgUCGCCa -3' miRNA: 3'- cugCUUGCGa-AgUGGCuuCCA-GGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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