Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7339 | 3' | -56.2 | NC_001900.1 | + | 29315 | 0.67 | 0.634587 |
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Target: 5'- uCCUCGaacgAGuaGUCCGGGaUGACGa-- -3' miRNA: 3'- -GGAGCa---UCcgCAGGCCCaGCUGCaac -5' |
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| 7339 | 3' | -56.2 | NC_001900.1 | + | 32332 | 0.67 | 0.612967 |
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Target: 5'- cCCUU---GGUGUCCuuGUCGACGUUGa -3' miRNA: 3'- -GGAGcauCCGCAGGccCAGCUGCAAC- -5' |
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| 7339 | 3' | -56.2 | NC_001900.1 | + | 45178 | 0.68 | 0.538236 |
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Target: 5'- -gUCGUAGGU--CCGGuuccaguagaaGUCGACGUUGa -3' miRNA: 3'- ggAGCAUCCGcaGGCC-----------CAGCUGCAAC- -5' |
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| 7339 | 3' | -56.2 | NC_001900.1 | + | 46676 | 0.69 | 0.527783 |
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Target: 5'- --aUGUAGGCGUCCaGGUUGGCc--- -3' miRNA: 3'- ggaGCAUCCGCAGGcCCAGCUGcaac -5' |
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| 7339 | 3' | -56.2 | NC_001900.1 | + | 4934 | 0.7 | 0.428341 |
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Target: 5'- aCCUCGgguucAGGCGacucccUCCGGGa-GGCGUUGc -3' miRNA: 3'- -GGAGCa----UCCGC------AGGCCCagCUGCAAC- -5' |
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| 7339 | 3' | -56.2 | NC_001900.1 | + | 12003 | 0.71 | 0.400709 |
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Target: 5'- aCCgUGgcGGCGUCCGGGU-GACGc-- -3' miRNA: 3'- -GGaGCauCCGCAGGCCCAgCUGCaac -5' |
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| 7339 | 3' | -56.2 | NC_001900.1 | + | 32425 | 0.71 | 0.400709 |
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Target: 5'- gCCUUGgccUGGGCcgcGUUCaGGUCGGCGUUGa -3' miRNA: 3'- -GGAGC---AUCCG---CAGGcCCAGCUGCAAC- -5' |
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| 7339 | 3' | -56.2 | NC_001900.1 | + | 40536 | 0.75 | 0.241316 |
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Target: 5'- aUCUUGUcgAGGUacuuGUCCGGGUCGGCGa-- -3' miRNA: 3'- -GGAGCA--UCCG----CAGGCCCAGCUGCaac -5' |
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| 7339 | 3' | -56.2 | NC_001900.1 | + | 33647 | 1.11 | 0.000686 |
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Target: 5'- cCCUCGUAGGCGUCCGGGUCGACGUUGg -3' miRNA: 3'- -GGAGCAUCCGCAGGCCCAGCUGCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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