Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7344 | 5' | -64.6 | NC_001900.1 | + | 35180 | 1.03 | 0.000258 |
Target: 5'- gUUCGCCCCGACGCCACCGCCCUCCAUg -3' miRNA: 3'- -AAGCGGGGCUGCGGUGGCGGGAGGUA- -5' |
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7344 | 5' | -64.6 | NC_001900.1 | + | 36181 | 0.68 | 0.172598 |
Target: 5'- aUCGCCUCGGCaGCCgaACCcCUCUCCGg -3' miRNA: 3'- aAGCGGGGCUG-CGG--UGGcGGGAGGUa -5' |
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7344 | 5' | -64.6 | NC_001900.1 | + | 33300 | 0.67 | 0.181846 |
Target: 5'- -gCGUCggCCGGCGCagGCCGCaCCUCCAc -3' miRNA: 3'- aaGCGG--GGCUGCGg-UGGCG-GGAGGUa -5' |
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7344 | 5' | -64.6 | NC_001900.1 | + | 1357 | 0.67 | 0.206904 |
Target: 5'- -aCGCgCUGGCgGUCGCCGCCCgcgaCCAg -3' miRNA: 3'- aaGCGgGGCUG-CGGUGGCGGGa---GGUa -5' |
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7344 | 5' | -64.6 | NC_001900.1 | + | 25758 | 0.66 | 0.246948 |
Target: 5'- -gCGCCCCGGCGCaCGCgGagCUUCAUc -3' miRNA: 3'- aaGCGGGGCUGCG-GUGgCggGAGGUA- -5' |
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7344 | 5' | -64.6 | NC_001900.1 | + | 47824 | 0.66 | 0.246948 |
Target: 5'- --aGCUCCGcUGCCGCCGCCa-CCGa -3' miRNA: 3'- aagCGGGGCuGCGGUGGCGGgaGGUa -5' |
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7344 | 5' | -64.6 | NC_001900.1 | + | 38019 | 0.67 | 0.212261 |
Target: 5'- -gCGCCCgGugGCgACCcucgcccaGCCCUUCAc -3' miRNA: 3'- aaGCGGGgCugCGgUGG--------CGGGAGGUa -5' |
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7344 | 5' | -64.6 | NC_001900.1 | + | 1459 | 0.67 | 0.212261 |
Target: 5'- -aCGCUCCGAagguuCGCCACC-CCCggCCGa -3' miRNA: 3'- aaGCGGGGCU-----GCGGUGGcGGGa-GGUa -5' |
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7344 | 5' | -64.6 | NC_001900.1 | + | 36051 | 0.68 | 0.168133 |
Target: 5'- --aGCCCuCGcCGCCGCCGCUgaCCAc -3' miRNA: 3'- aagCGGG-GCuGCGGUGGCGGgaGGUa -5' |
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7344 | 5' | -64.6 | NC_001900.1 | + | 1766 | 0.69 | 0.151293 |
Target: 5'- -cUGCCCgGACGCgACCGCgUUCUGUg -3' miRNA: 3'- aaGCGGGgCUGCGgUGGCGgGAGGUA- -5' |
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7344 | 5' | -64.6 | NC_001900.1 | + | 7283 | 0.71 | 0.095622 |
Target: 5'- aUCGCCaCCG-CaGCCAUCGCCCUCg-- -3' miRNA: 3'- aAGCGG-GGCuG-CGGUGGCGGGAGgua -5' |
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7344 | 5' | -64.6 | NC_001900.1 | + | 47882 | 0.71 | 0.095622 |
Target: 5'- -cCGCCCCGGCcgcuuccggcgGcCCACCGCcgCCUCCAa -3' miRNA: 3'- aaGCGGGGCUG-----------C-GGUGGCG--GGAGGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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