Results 21 - 40 of 57 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7348 | 3' | -51.4 | NC_001900.1 | + | 29705 | 0.67 | 0.828341 |
Target: 5'- -aGCGCCaGGuacagguACCGgguacCGGAGUCCGGGg -3' miRNA: 3'- agUGCGG-CUu------UGGCa----GCUUCAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 4579 | 0.67 | 0.828341 |
Target: 5'- -aGCGCCGGuacACCGUCGGccuGGagCAGGu -3' miRNA: 3'- agUGCGGCUu--UGGCAGCU---UCagGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 45275 | 0.68 | 0.818942 |
Target: 5'- gUCACGCCGA--CCG-CGAAGgugacgacgCCGAc -3' miRNA: 3'- -AGUGCGGCUuuGGCaGCUUCa--------GGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 9608 | 0.68 | 0.81799 |
Target: 5'- gUACGCCGAGAUCGauccucggaucggUCGGccGG-CCAAGg -3' miRNA: 3'- aGUGCGGCUUUGGC-------------AGCU--UCaGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 42429 | 0.68 | 0.809336 |
Target: 5'- aUCugGgUGAAcgCGUCGGAGUCCGc- -3' miRNA: 3'- -AGugCgGCUUugGCAGCUUCAGGUuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 2972 | 0.68 | 0.789547 |
Target: 5'- uUCGCGgCGAAGCCGUUGGua--CGGGg -3' miRNA: 3'- -AGUGCgGCUUUGGCAGCUucagGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 45222 | 0.68 | 0.789547 |
Target: 5'- cCGCGCCGAucagcccggagaAGCCGUUGAagaAGUUCu-- -3' miRNA: 3'- aGUGCGGCU------------UUGGCAGCU---UCAGGuuc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 12187 | 0.69 | 0.769068 |
Target: 5'- --uCGCCGAGGCCGagaagaccUCGAucGUCCAGc -3' miRNA: 3'- aguGCGGCUUUGGC--------AGCUu-CAGGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 5593 | 0.69 | 0.769068 |
Target: 5'- uUCAccCGCUGGAGCUGUCGGAGcugcgCUggGc -3' miRNA: 3'- -AGU--GCGGCUUUGGCAGCUUCa----GGuuC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 8187 | 0.69 | 0.765942 |
Target: 5'- --uCGCCGAGAaggcguaCGUCGAguggcaggacgugcAGUCCGGGa -3' miRNA: 3'- aguGCGGCUUUg------GCAGCU--------------UCAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 18107 | 0.69 | 0.758599 |
Target: 5'- -aGCGCCGAcuuCCGUUGccgccuAGUCCAGu -3' miRNA: 3'- agUGCGGCUuu-GGCAGCu-----UCAGGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 3440 | 0.69 | 0.752251 |
Target: 5'- cCACGCUGGccuGGCCGUggcagaCGAcgaaguuucaggggaAGUCCAAGg -3' miRNA: 3'- aGUGCGGCU---UUGGCA------GCU---------------UCAGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 15829 | 0.69 | 0.747993 |
Target: 5'- ---gGUCGAGGCCGUCaagGAGGUCgAGGa -3' miRNA: 3'- agugCGGCUUUGGCAG---CUUCAGgUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 37261 | 0.69 | 0.74157 |
Target: 5'- -gACGCCGAcagGGCCGUCGuacacggcccgcagcGGGgCCAGGa -3' miRNA: 3'- agUGCGGCU---UUGGCAGC---------------UUCaGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 47348 | 0.69 | 0.737264 |
Target: 5'- cUCAUGCCcuugggcuuGAcGCCGUCGAAcUCCAGc -3' miRNA: 3'- -AGUGCGG---------CUuUGGCAGCUUcAGGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 19545 | 0.7 | 0.704457 |
Target: 5'- aCAcCGCCGugGCCGUgGGAGgaacugcCCAAGg -3' miRNA: 3'- aGU-GCGGCuuUGGCAgCUUCa------GGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 4405 | 0.7 | 0.682192 |
Target: 5'- cUguCGCCGAGGCgGUCGAGGUaUggGa -3' miRNA: 3'- -AguGCGGCUUUGgCAGCUUCAgGuuC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 24153 | 0.7 | 0.670979 |
Target: 5'- -aGCGCC---GCCGUCGuAGGUCCGGu -3' miRNA: 3'- agUGCGGcuuUGGCAGC-UUCAGGUUc -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 47220 | 0.7 | 0.670979 |
Target: 5'- gCAgGCCGAGGCCGUCacccGGcuuUCCGGGg -3' miRNA: 3'- aGUgCGGCUUUGGCAGcu--UC---AGGUUC- -5' |
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7348 | 3' | -51.4 | NC_001900.1 | + | 23351 | 0.71 | 0.659727 |
Target: 5'- aUCGuCGUCGGcaacaccaaGACCGUC-AGGUCCAAGc -3' miRNA: 3'- -AGU-GCGGCU---------UUGGCAGcUUCAGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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