Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7351 | 3' | -52.1 | NC_001900.1 | + | 19840 | 0.66 | 0.862324 |
Target: 5'- aCACCGACcgaCGCGAggagcagaucaGCUCCGagUCGg -3' miRNA: 3'- gGUGGCUGaa-GCGCU-----------UGAGGUauGGC- -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 38343 | 1.12 | 0.001412 |
Target: 5'- gCCACCGACUUCGCGAACUCCAUACCGg -3' miRNA: 3'- -GGUGGCUGAAGCGCUUGAGGUAUGGC- -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 41004 | 0.72 | 0.56089 |
Target: 5'- uCCGCUGcGCggguaGCGGACUCCGUACUu -3' miRNA: 3'- -GGUGGC-UGaag--CGCUUGAGGUAUGGc -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 45055 | 0.71 | 0.637428 |
Target: 5'- aCAUCGGCUUCgugucucggcacaGCGGGCUCCgGUACgCGa -3' miRNA: 3'- gGUGGCUGAAG-------------CGCUUGAGG-UAUG-GC- -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 13645 | 0.7 | 0.660823 |
Target: 5'- gCCAgCGACgUUCaGCGGGCUCCgaucaaGUACCc -3' miRNA: 3'- -GGUgGCUG-AAG-CGCUUGAGG------UAUGGc -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 6817 | 0.69 | 0.715845 |
Target: 5'- aCGCgGACUUCGCGAuggcggguuACUCgCAgggaGCCa -3' miRNA: 3'- gGUGgCUGAAGCGCU---------UGAG-GUa---UGGc -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 15111 | 0.69 | 0.737342 |
Target: 5'- gCCACCGGCUgguCGAGCgucggCCAcACCa -3' miRNA: 3'- -GGUGGCUGAagcGCUUGa----GGUaUGGc -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 23323 | 0.69 | 0.737342 |
Target: 5'- gCC-CCGGCUggGcCGAGUUCUAUACCGg -3' miRNA: 3'- -GGuGGCUGAagC-GCUUGAGGUAUGGC- -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 46098 | 0.68 | 0.762554 |
Target: 5'- aCCAaCGGCUUCGCcgcgaagguagauguGAcCUCCAUcGCCGa -3' miRNA: 3'- -GGUgGCUGAAGCG---------------CUuGAGGUA-UGGC- -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 7740 | 0.68 | 0.768741 |
Target: 5'- aCCGCCGcGCUgugucUCGcCGAACUCUGUgGCCc -3' miRNA: 3'- -GGUGGC-UGA-----AGC-GCUUGAGGUA-UGGc -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 32680 | 0.67 | 0.81803 |
Target: 5'- --uCCGGCca-GCGAACUCCGcgaguucgucUGCCGa -3' miRNA: 3'- gguGGCUGaagCGCUUGAGGU----------AUGGC- -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 4984 | 0.67 | 0.830991 |
Target: 5'- aCC-CCGGCaugcUCGuCGGGCUCCAgcacggcggcggggGCCGg -3' miRNA: 3'- -GGuGGCUGa---AGC-GCUUGAGGUa-------------UGGC- -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 44460 | 0.67 | 0.836418 |
Target: 5'- gCCACgCGAagaaCUUCuCGAccuGCUCCAgGCCGa -3' miRNA: 3'- -GGUG-GCU----GAAGcGCU---UGAGGUaUGGC- -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 32961 | 0.67 | 0.845286 |
Target: 5'- aCACCgGACU---UGAACUCCAUcuuGCCGu -3' miRNA: 3'- gGUGG-CUGAagcGCUUGAGGUA---UGGC- -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 17187 | 0.66 | 0.853924 |
Target: 5'- uUCACCGACguggugUCGCaggguccgGGGCUCCAgcagcuCCa -3' miRNA: 3'- -GGUGGCUGa-----AGCG--------CUUGAGGUau----GGc -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 21057 | 0.68 | 0.768741 |
Target: 5'- aCACCGGCccgaacggCGUGAuCUUCGUGCCc -3' miRNA: 3'- gGUGGCUGaa------GCGCUuGAGGUAUGGc -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 31570 | 0.73 | 0.486042 |
Target: 5'- aCCACCGAgCUgacccaUCGCcugcagaccGGACUCCAUgaGCCGg -3' miRNA: 3'- -GGUGGCU-GA------AGCG---------CUUGAGGUA--UGGC- -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 10006 | 0.71 | 0.582913 |
Target: 5'- gCCACCGGCaUCGCGGAggUCAU-CCa -3' miRNA: 3'- -GGUGGCUGaAGCGCUUgaGGUAuGGc -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 47806 | 0.71 | 0.582913 |
Target: 5'- aCCACCGAacccgcCGCcAGCUCCGcUGCCGc -3' miRNA: 3'- -GGUGGCUgaa---GCGcUUGAGGU-AUGGC- -5' |
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7351 | 3' | -52.1 | NC_001900.1 | + | 35217 | 0.7 | 0.694009 |
Target: 5'- gCACCGGCUgguacCGGGCUCCgGUAUCGa -3' miRNA: 3'- gGUGGCUGAagc--GCUUGAGG-UAUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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