Results 21 - 34 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7353 | 5' | -54.9 | NC_001900.1 | + | 17492 | 0.67 | 0.668448 |
Target: 5'- cGAGCGUGA--GGU-CCGGGa-CAUCCg -3' miRNA: 3'- -CUCGCGCUugUCGuGGCCCaaGUAGG- -5' |
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7353 | 5' | -54.9 | NC_001900.1 | + | 43130 | 0.66 | 0.679496 |
Target: 5'- aGAGCauCGAGCuGCGCuCGGGUgCcgCCu -3' miRNA: 3'- -CUCGc-GCUUGuCGUG-GCCCAaGuaGG- -5' |
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7353 | 5' | -54.9 | NC_001900.1 | + | 35830 | 0.66 | 0.679496 |
Target: 5'- -uGUGgGAACAGCuCCGGGaaCA-CCu -3' miRNA: 3'- cuCGCgCUUGUCGuGGCCCaaGUaGG- -5' |
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7353 | 5' | -54.9 | NC_001900.1 | + | 40201 | 0.66 | 0.690497 |
Target: 5'- -cGCGCaugucGAACGcCACCGGGUUguUCg -3' miRNA: 3'- cuCGCG-----CUUGUcGUGGCCCAAguAGg -5' |
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7353 | 5' | -54.9 | NC_001900.1 | + | 34037 | 0.66 | 0.701441 |
Target: 5'- -uGCGCuucuuGAcCGGCACCGGG---AUCCg -3' miRNA: 3'- cuCGCG-----CUuGUCGUGGCCCaagUAGG- -5' |
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7353 | 5' | -54.9 | NC_001900.1 | + | 35905 | 0.66 | 0.712317 |
Target: 5'- cAGcCGCGAucaGGCGCUGGGcgucUCGUCg -3' miRNA: 3'- cUC-GCGCUug-UCGUGGCCCa---AGUAGg -5' |
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7353 | 5' | -54.9 | NC_001900.1 | + | 29704 | 0.66 | 0.712317 |
Target: 5'- cAGCGCcagguACAgGUACCGGGUaccggaGUCCg -3' miRNA: 3'- cUCGCGcu---UGU-CGUGGCCCAag----UAGG- -5' |
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7353 | 5' | -54.9 | NC_001900.1 | + | 28978 | 0.66 | 0.712317 |
Target: 5'- -cGCGCuuGCAGCGCCGcaguuGGaUCuUCCg -3' miRNA: 3'- cuCGCGcuUGUCGUGGC-----CCaAGuAGG- -5' |
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7353 | 5' | -54.9 | NC_001900.1 | + | 29363 | 0.66 | 0.712317 |
Target: 5'- cGGUGcCGAuCAGCACCGGaccCAUCUc -3' miRNA: 3'- cUCGC-GCUuGUCGUGGCCcaaGUAGG- -5' |
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7353 | 5' | -54.9 | NC_001900.1 | + | 29539 | 0.66 | 0.716645 |
Target: 5'- cGAGaCGUGGACuggaucugguaguucAGCGCCGaGUaCGUCCg -3' miRNA: 3'- -CUC-GCGCUUG---------------UCGUGGCcCAaGUAGG- -5' |
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7353 | 5' | -54.9 | NC_001900.1 | + | 29843 | 0.66 | 0.723111 |
Target: 5'- cAGCGCGAGCuGuCAUCaGGUggagGUCCg -3' miRNA: 3'- cUCGCGCUUGuC-GUGGcCCAag--UAGG- -5' |
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7353 | 5' | -54.9 | NC_001900.1 | + | 22823 | 0.66 | 0.723111 |
Target: 5'- cGAGCGCGAGaagaaGGCugCGGcgaGUgcCAUCa -3' miRNA: 3'- -CUCGCGCUUg----UCGugGCC---CAa-GUAGg -5' |
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7353 | 5' | -54.9 | NC_001900.1 | + | 26870 | 0.66 | 0.723111 |
Target: 5'- -uGUGCGAGCAuCGCUcGGUUC-UCCu -3' miRNA: 3'- cuCGCGCUUGUcGUGGcCCAAGuAGG- -5' |
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7353 | 5' | -54.9 | NC_001900.1 | + | 41204 | 0.66 | 0.723111 |
Target: 5'- cAGCGCGccCAGCACCugcuggugcagcGGGUUgG-CCu -3' miRNA: 3'- cUCGCGCuuGUCGUGG------------CCCAAgUaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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