Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7360 | 3' | -57.1 | NC_001900.1 | + | 40444 | 0.66 | 0.660263 |
Target: 5'- aGCCGAccagAGCCGUCC-AgUCGU--UGGa -3' miRNA: 3'- cCGGCU----UCGGCAGGcUgAGCGcuACC- -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 1586 | 0.66 | 0.659191 |
Target: 5'- gGGCCGGAucuccucGCUGUUCGAggucggcuuCUCGgcCGGUGGc -3' miRNA: 3'- -CCGGCUU-------CGGCAGGCU---------GAGC--GCUACC- -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 15061 | 0.66 | 0.650604 |
Target: 5'- cGGCCGAAcugaagaccaucgacCUGUCCGACcCGuCGAccUGGa -3' miRNA: 3'- -CCGGCUUc--------------GGCAGGCUGaGC-GCU--ACC- -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 27469 | 0.66 | 0.646307 |
Target: 5'- gGGCCGAgccgcacggccuugAGCCGgCCGuCg-GCGAUGu -3' miRNA: 3'- -CCGGCU--------------UCGGCaGGCuGagCGCUACc -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 24437 | 0.66 | 0.628027 |
Target: 5'- cGCCGAAGCCGcCUuGGCcccaCGCaGUGGc -3' miRNA: 3'- cCGGCUUCGGCaGG-CUGa---GCGcUACC- -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 22975 | 0.66 | 0.628027 |
Target: 5'- cGCCGAGaugacccccGCCcuuGUCCGGg-CGUGGUGGg -3' miRNA: 3'- cCGGCUU---------CGG---CAGGCUgaGCGCUACC- -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 37263 | 0.66 | 0.617277 |
Target: 5'- cGCCGAcagGGCCGUCguacaCGGCcCGCaGcgGGg -3' miRNA: 3'- cCGGCU---UCGGCAG-----GCUGaGCG-CuaCC- -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 31663 | 0.66 | 0.617277 |
Target: 5'- -aCCGAAG--GUCCGcCUCGCGGUGu -3' miRNA: 3'- ccGGCUUCggCAGGCuGAGCGCUACc -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 41086 | 0.66 | 0.606538 |
Target: 5'- gGGCC-AGGCCGg-UGAcCUCGCGGccgUGGa -3' miRNA: 3'- -CCGGcUUCGGCagGCU-GAGCGCU---ACC- -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 40324 | 0.66 | 0.606538 |
Target: 5'- cGGCCGugguuGGCCGcgUCGAg-UGCGAUGa -3' miRNA: 3'- -CCGGCu----UCGGCa-GGCUgaGCGCUACc -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 12746 | 0.67 | 0.585133 |
Target: 5'- gGGCCGGAucgucGcCCGUCCGACcauccUCaGCGAccacgucgUGGa -3' miRNA: 3'- -CCGGCUU-----C-GGCAGGCUG-----AG-CGCU--------ACC- -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 18510 | 0.67 | 0.574483 |
Target: 5'- cGGCUG-AGCUGUCU--CagGCGAUGGa -3' miRNA: 3'- -CCGGCuUCGGCAGGcuGagCGCUACC- -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 15274 | 0.67 | 0.568114 |
Target: 5'- uGGUCGAGGCCGUggccgcacgguucggCCGAgaCGcCGA-GGa -3' miRNA: 3'- -CCGGCUUCGGCA---------------GGCUgaGC-GCUaCC- -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 9138 | 0.67 | 0.563878 |
Target: 5'- gGGCCGGgugguGGCCGUCCGAUgacaGCc---- -3' miRNA: 3'- -CCGGCU-----UCGGCAGGCUGag--CGcuacc -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 45268 | 0.67 | 0.542832 |
Target: 5'- cGUCGAGGUCacgCCGAC-CGCGAaGGu -3' miRNA: 3'- cCGGCUUCGGca-GGCUGaGCGCUaCC- -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 10218 | 0.68 | 0.491487 |
Target: 5'- uGCaGAGGCUGUCCGAaUCGCaGAgGGa -3' miRNA: 3'- cCGgCUUCGGCAGGCUgAGCG-CUaCC- -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 5599 | 0.68 | 0.491487 |
Target: 5'- cGCUGGAGCUGUCgGAgcUGCGcUGGg -3' miRNA: 3'- cCGGCUUCGGCAGgCUgaGCGCuACC- -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 16785 | 0.68 | 0.491487 |
Target: 5'- cGGCUcGGGCCGUucugCCGAacaugaccaaCUCGCcGAUGGu -3' miRNA: 3'- -CCGGcUUCGGCA----GGCU----------GAGCG-CUACC- -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 34869 | 0.68 | 0.490482 |
Target: 5'- cGGCCcaGAGGCCGUgCCGccgaacgucuucaGCUUGCGAc-- -3' miRNA: 3'- -CCGG--CUUCGGCA-GGC-------------UGAGCGCUacc -5' |
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7360 | 3' | -57.1 | NC_001900.1 | + | 21591 | 0.69 | 0.481482 |
Target: 5'- cGGCCGGcucaaGGCCGUgCGGCUCGg----- -3' miRNA: 3'- -CCGGCU-----UCGGCAgGCUGAGCgcuacc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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