Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7366 | 3' | -58.8 | NC_001900.1 | + | 33778 | 0.67 | 0.507171 |
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Target: 5'- gGcGUCUCgGCCGaacCGuGCggCCACGGCCu -3' miRNA: 3'- gC-CAGAGgUGGCa--GCuCG--GGUGCUGG- -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 6502 | 0.66 | 0.51731 |
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Target: 5'- gCGGUCccCCAgC-UCG-GCCCGCuGGCCa -3' miRNA: 3'- -GCCAGa-GGUgGcAGCuCGGGUG-CUGG- -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 29677 | 0.66 | 0.527529 |
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Target: 5'- cCGGgauaCGCCuuGUCGAacccGUCCACGACCu -3' miRNA: 3'- -GCCagagGUGG--CAGCU----CGGGUGCUGG- -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 28118 | 0.66 | 0.527529 |
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Target: 5'- aGGUCUCC-UUGUCGAGUUguCGuuCCa -3' miRNA: 3'- gCCAGAGGuGGCAGCUCGGguGCu-GG- -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 7453 | 0.66 | 0.527529 |
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Target: 5'- uCGGUgCUCCA---UCGAGCCCGacACCg -3' miRNA: 3'- -GCCA-GAGGUggcAGCUCGGGUgcUGG- -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 44061 | 0.66 | 0.548183 |
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Target: 5'- cCGGcCcccgCCGCCGUgcugGAGCCCgACGAgCa -3' miRNA: 3'- -GCCaGa---GGUGGCAg---CUCGGG-UGCUgG- -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 6986 | 0.66 | 0.548183 |
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Target: 5'- aCGGgCUCCACCacGUCaccGGCCCguacaACGACg -3' miRNA: 3'- -GCCaGAGGUGG--CAGc--UCGGG-----UGCUGg -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 30057 | 0.66 | 0.548183 |
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Target: 5'- uGGUuacCUCCugguggaUGUCaagcucGGCCCACGACCg -3' miRNA: 3'- gCCA---GAGGug-----GCAGc-----UCGGGUGCUGG- -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 33816 | 0.66 | 0.548183 |
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Target: 5'- aGGUgUCCuuCGUCGGGaUCuCGACCa -3' miRNA: 3'- gCCAgAGGugGCAGCUCgGGuGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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