Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7366 | 3' | -58.8 | NC_001900.1 | + | 26128 | 0.69 | 0.388165 |
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Target: 5'- aCGGUCUCCagggcgcucgucagACCGUUgaccaGAGCCUgggggaucuccccgAUGACCu -3' miRNA: 3'- -GCCAGAGG--------------UGGCAG-----CUCGGG--------------UGCUGG- -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 32023 | 0.69 | 0.376106 |
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Target: 5'- uCGG-CU-CGCCGUUGAGCaCCuugguaGCGGCCg -3' miRNA: 3'- -GCCaGAgGUGGCAGCUCG-GG------UGCUGG- -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 41604 | 0.69 | 0.367649 |
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Target: 5'- uGGgCUCCGgUGUCGGGCUCGauggagcacCGACCc -3' miRNA: 3'- gCCaGAGGUgGCAGCUCGGGU---------GCUGG- -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 40444 | 0.69 | 0.351131 |
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Target: 5'- cCGGUgC-CUACCGUUGAGaCCC-CGACg -3' miRNA: 3'- -GCCA-GaGGUGGCAGCUC-GGGuGCUGg -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 14246 | 0.7 | 0.31218 |
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Target: 5'- aGGUCUCCgagauccugaGCCGgUGuGCCCGCGAa- -3' miRNA: 3'- gCCAGAGG----------UGGCaGCuCGGGUGCUgg -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 45704 | 0.71 | 0.269217 |
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Target: 5'- cCGGUCUCCAguUCGagGAacaggucGCCCGCaGGCCa -3' miRNA: 3'- -GCCAGAGGU--GGCagCU-------CGGGUG-CUGG- -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 12408 | 0.72 | 0.244328 |
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Target: 5'- aGGUCUUCGCCGagGAGaucaCCCGCaACCu -3' miRNA: 3'- gCCAGAGGUGGCagCUC----GGGUGcUGG- -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 20711 | 0.72 | 0.238262 |
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Target: 5'- aGGUCUUCGCUGUCGA-CCa--GACCg -3' miRNA: 3'- gCCAGAGGUGGCAGCUcGGgugCUGG- -5' |
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| 7366 | 3' | -58.8 | NC_001900.1 | + | 44857 | 1.11 | 0.000359 |
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Target: 5'- gCGGUCUCCACCGUCGAGCCCACGACCu -3' miRNA: 3'- -GCCAGAGGUGGCAGCUCGGGUGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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