Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
743 | 5' | -61.5 | AC_000020.1 | + | 7973 | 0.66 | 0.257173 |
Target: 5'- -aGGCcuGUGCugauccugaGCUGGgaGGCUagGGCCa -3' miRNA: 3'- ccCCG--CACG---------UGACCgaCCGGuaCCGG- -5' |
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743 | 5' | -61.5 | AC_000020.1 | + | 16739 | 0.66 | 0.257173 |
Target: 5'- cGGGGgGgaguagGCugGC-GGCUGGUCAgagGGCUu -3' miRNA: 3'- -CCCCgCa-----CG--UGaCCGACCGGUa--CCGG- -5' |
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743 | 5' | -61.5 | AC_000020.1 | + | 8857 | 0.67 | 0.231731 |
Target: 5'- cGGGGUGcaggGCGCgGGuCUGGgCA-GGCUc -3' miRNA: 3'- -CCCCGCa---CGUGaCC-GACCgGUaCCGG- -5' |
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743 | 5' | -61.5 | AC_000020.1 | + | 30179 | 0.67 | 0.202945 |
Target: 5'- aGGGGCccGCugUGGCUGGaaa--GCCu -3' miRNA: 3'- -CCCCGcaCGugACCGACCgguacCGG- -5' |
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743 | 5' | -61.5 | AC_000020.1 | + | 17713 | 0.68 | 0.197574 |
Target: 5'- aGGG-GUGCAagGGCUgGGCCAgcaGGCUg -3' miRNA: 3'- cCCCgCACGUgaCCGA-CCGGUa--CCGG- -5' |
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743 | 5' | -61.5 | AC_000020.1 | + | 30247 | 0.69 | 0.163352 |
Target: 5'- cGGGCuGUGCGCaGGCUuuccaGCCAcagcgGGCCc -3' miRNA: 3'- cCCCG-CACGUGaCCGAc----CGGUa----CCGG- -5' |
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743 | 5' | -61.5 | AC_000020.1 | + | 24519 | 0.69 | 0.145872 |
Target: 5'- uGGGGCG-GCuucCUGaacaagcauggcaGCUGGCCAaauUGGCg -3' miRNA: 3'- -CCCCGCaCGu--GAC-------------CGACCGGU---ACCGg -5' |
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743 | 5' | -61.5 | AC_000020.1 | + | 14377 | 0.7 | 0.123705 |
Target: 5'- cGGGGCGUGcCAUga--UGGCCAUGcGCg -3' miRNA: 3'- -CCCCGCAC-GUGaccgACCGGUAC-CGg -5' |
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743 | 5' | -61.5 | AC_000020.1 | + | 3189 | 0.75 | 0.055329 |
Target: 5'- aGGGGCGcgGUACgUGGCUGuguauuuacaGCCAUGGaCCc -3' miRNA: 3'- -CCCCGCa-CGUG-ACCGAC----------CGGUACC-GG- -5' |
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743 | 5' | -61.5 | AC_000020.1 | + | 19800 | 1.12 | 5.8e-05 |
Target: 5'- gGGGGCGUGCACUGGCUGGCCAUGGCCu -3' miRNA: 3'- -CCCCGCACGUGACCGACCGGUACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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