Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7437 | 3' | -50.7 | NC_001902.1 | + | 22077 | 0.95 | 0.010718 |
Target: 5'- aAUGAAGUGGUugGGGAUGAGAACUCCa -3' miRNA: 3'- -UACUUCACCAcaCCCUACUCUUGAGG- -5' |
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7437 | 3' | -50.7 | NC_001902.1 | + | 8586 | 0.71 | 0.426204 |
Target: 5'- -aGAAGgGGUGgGGGAUauggGGGAAUUCCu -3' miRNA: 3'- uaCUUCaCCACaCCCUA----CUCUUGAGG- -5' |
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7437 | 3' | -50.7 | NC_001902.1 | + | 19563 | 0.68 | 0.63339 |
Target: 5'- -gGAGGUGcGUGUGGGGU----ACUCUg -3' miRNA: 3'- uaCUUCAC-CACACCCUAcucuUGAGG- -5' |
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7437 | 3' | -50.7 | NC_001902.1 | + | 21981 | 0.67 | 0.68158 |
Target: 5'- -gGAAGcucgGGUG-GGGGUGGGGuauggACUCUc -3' miRNA: 3'- uaCUUCa---CCACaCCCUACUCU-----UGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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