Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 744 | 3' | -58 | AC_000020.1 | + | 31177 | 0.67 | 0.361643 |
|
Target: 5'- uUC-CCGUGGGAacaCCCUgAccAGGGCGUg -3' miRNA: 3'- gGGuGGUACCCUg--GGGAgU--UCUCGCA- -5' |
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| 744 | 3' | -58 | AC_000020.1 | + | 20080 | 1.09 | 0.000261 |
|
Target: 5'- uCCCACCAUGGGACCCCUCAAGAGCGUg -3' miRNA: 3'- -GGGUGGUACCCUGGGGAGUUCUCGCA- -5' |
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| 744 | 3' | -58 | AC_000020.1 | + | 15314 | 0.67 | 0.361643 |
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Target: 5'- gCCUcaACC-UGGGGCUCCUCA-GAGUa- -3' miRNA: 3'- -GGG--UGGuACCCUGGGGAGUuCUCGca -5' |
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| 744 | 3' | -58 | AC_000020.1 | + | 14792 | 0.66 | 0.407204 |
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Target: 5'- cCCCGCCAcugguaGGGACgCCg-GGGAGCa- -3' miRNA: 3'- -GGGUGGUa-----CCCUGgGGagUUCUCGca -5' |
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| 744 | 3' | -58 | AC_000020.1 | + | 14425 | 0.66 | 0.397807 |
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Target: 5'- gCCAUCAUGGcacGCCCCgCAGGGGUu- -3' miRNA: 3'- gGGUGGUACCc--UGGGGaGUUCUCGca -5' |
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| 744 | 3' | -58 | AC_000020.1 | + | 7131 | 0.67 | 0.352107 |
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Target: 5'- gCCACUAcuuUGGGACuagaaagCCCUgAAGAGCu- -3' miRNA: 3'- gGGUGGU---ACCCUG-------GGGAgUUCUCGca -5' |
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| 744 | 3' | -58 | AC_000020.1 | + | 147 | 0.67 | 0.361643 |
|
Target: 5'- uUC-CCGUGGGAacaCCCUgAccAGGGCGUg -3' miRNA: 3'- gGGuGGUACCCUg--GGGAgU--UCUCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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