Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7531 | 3' | -54.5 | NC_001944.1 | + | 1165 | 0.66 | 0.056571 |
Target: 5'- cCCGacacGACGaaCACGGUAGCGACgaaGGCgCCa -3' miRNA: 3'- -GGC----CUGUa-GUGCCGUCGUUG---UCGaGG- -5' |
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7531 | 3' | -54.5 | NC_001944.1 | + | 1903 | 0.66 | 0.050478 |
Target: 5'- aUGuGACGUCuGCGGUAGUAugGGCgggcauaCCg -3' miRNA: 3'- gGC-CUGUAG-UGCCGUCGUugUCGa------GG- -5' |
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7531 | 3' | -54.5 | NC_001944.1 | + | 362 | 0.66 | 0.048593 |
Target: 5'- --uGACcUCGCGGCAGCAuuuucuuaaGCGcGCUCa -3' miRNA: 3'- ggcCUGuAGUGCCGUCGU---------UGU-CGAGg -5' |
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7531 | 3' | -54.5 | NC_001944.1 | + | 68 | 0.68 | 0.035792 |
Target: 5'- gCGGGCAccucCGCaaGGCAGCcAAUAGCUgCg -3' miRNA: 3'- gGCCUGUa---GUG--CCGUCG-UUGUCGAgG- -5' |
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7531 | 3' | -54.5 | NC_001944.1 | + | 501 | 1.14 | 2e-06 |
Target: 5'- uCCGGACAUCACGGCAGCAACAGCUCCa -3' miRNA: 3'- -GGCCUGUAGUGCCGUCGUUGUCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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