Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7634 | 3' | -53.7 | NC_001973.1 | + | 8170 | 0.66 | 0.95804 |
Target: 5'- cCGUCGGC--GGGUCGAcggaCGAGcgCGc -3' miRNA: 3'- -GCAGCCGauCCCAGCUa---GCUCaaGUu -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 160827 | 0.69 | 0.889074 |
Target: 5'- gCGUCGGCcGGaGUCGAucaUCGAGgUCGAg -3' miRNA: 3'- -GCAGCCGaUCcCAGCU---AGCUCaAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 127383 | 0.69 | 0.889074 |
Target: 5'- aCGUgcCGGCaAGGGgCaAUCGAGUUCAAa -3' miRNA: 3'- -GCA--GCCGaUCCCaGcUAGCUCAAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 151380 | 0.69 | 0.874878 |
Target: 5'- gCGUCGGCcGGGGUCG-UCuGAGgUUAGa -3' miRNA: 3'- -GCAGCCGaUCCCAGCuAG-CUCaAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 129332 | 0.72 | 0.763371 |
Target: 5'- gCGcCGGCaAGGGUCGAUaGAGgUCAAg -3' miRNA: 3'- -GCaGCCGaUCCCAGCUAgCUCaAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 160491 | 0.74 | 0.652613 |
Target: 5'- aCGUgcCGGCcAGGGUCGAUgGAGgUCGAg -3' miRNA: 3'- -GCA--GCCGaUCCCAGCUAgCUCaAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 130645 | 0.74 | 0.631903 |
Target: 5'- aCGUgcCGGCaAGGGUCaAUCGAGUUUAAg -3' miRNA: 3'- -GCA--GCCGaUCCCAGcUAGCUCAAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 129010 | 0.74 | 0.621544 |
Target: 5'- gCGUCGGCcAGGGUCGAUaaAGgUCAAa -3' miRNA: 3'- -GCAGCCGaUCCCAGCUAgcUCaAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 127539 | 0.74 | 0.621544 |
Target: 5'- aCGUgcCGGCaAGGGUCGAUgGAGgUCAAg -3' miRNA: 3'- -GCA--GCCGaUCCCAGCUAgCUCaAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 12141 | 0.76 | 0.519617 |
Target: 5'- gCGUCuGCcGGGGUCaAUCGAGUUCGAg -3' miRNA: 3'- -GCAGcCGaUCCCAGcUAGCUCAAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 128947 | 0.77 | 0.461617 |
Target: 5'- gCGcCGGCUAGGGUCGAU-GAGaUCAu -3' miRNA: 3'- -GCaGCCGAUCCCAGCUAgCUCaAGUu -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 160728 | 0.78 | 0.433921 |
Target: 5'- gCGcCGGCaAGGGUCaAUCGGGUUCAAg -3' miRNA: 3'- -GCaGCCGaUCCCAGcUAGCUCAAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 130726 | 0.78 | 0.424905 |
Target: 5'- gCGUCGGCcGGGG-CaAUCGAGUUCAAg -3' miRNA: 3'- -GCAGCCGaUCCCaGcUAGCUCAAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 127461 | 0.78 | 0.416002 |
Target: 5'- aCGUgcCGGCaAGGGUCaAUCGAGUUCAAa -3' miRNA: 3'- -GCA--GCCGaUCCCAGcUAGCUCAAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 72235 | 0.78 | 0.416002 |
Target: 5'- aCGUgcCGGCcGGGGUCaAUCGAGUUCAAg -3' miRNA: 3'- -GCA--GCCGaUCCCAGcUAGCUCAAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 16465 | 0.78 | 0.416002 |
Target: 5'- aCGUgcCGGCcGGGGUCaAUCGAGUUCAAg -3' miRNA: 3'- -GCA--GCCGaUCCCAGcUAGCUCAAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 12295 | 0.78 | 0.416002 |
Target: 5'- aCGUgcCGGCcAGGGUCaAUCGAGUUCAAa -3' miRNA: 3'- -GCA--GCCGaUCCCAGcUAGCUCAAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 130981 | 0.79 | 0.381561 |
Target: 5'- gCGUCGGCgAGGGUCGAUUgGAGgUCAAc -3' miRNA: 3'- -GCAGCCGaUCCCAGCUAG-CUCaAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 129176 | 0.8 | 0.326033 |
Target: 5'- gCGUCGGCaAGGGUCaAUgGAGUUCAAa -3' miRNA: 3'- -GCAGCCGaUCCCAGcUAgCUCAAGUU- -5' |
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7634 | 3' | -53.7 | NC_001973.1 | + | 129255 | 0.84 | 0.186536 |
Target: 5'- gCGUCGGCaAGGGUC-AUCGGGUUCAAg -3' miRNA: 3'- -GCAGCCGaUCCCAGcUAGCUCAAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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