Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7647 | 3' | -56.1 | NC_001973.1 | + | 100895 | 0.66 | 0.936338 |
Target: 5'- gACUuuGACCCuGUugugcgACAGGAUGUAgcuguugagacUGGUg -3' miRNA: 3'- -UGAggCUGGG-CA------UGUCCUGCAU-----------GCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 59543 | 0.66 | 0.931434 |
Target: 5'- cGCUUgGACgCGgccaAGGGCGUcGCGGUg -3' miRNA: 3'- -UGAGgCUGgGCaug-UCCUGCA-UGCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 138686 | 0.66 | 0.931434 |
Target: 5'- aGCUCCaucacgucuUCCGcggACAGGccuGCGUGCGGUg -3' miRNA: 3'- -UGAGGcu-------GGGCa--UGUCC---UGCAUGCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 70731 | 0.66 | 0.926299 |
Target: 5'- gAUUCCGACgacauugaCGaGCAGGACGaagucAUGGCa -3' miRNA: 3'- -UGAGGCUGg-------GCaUGUCCUGCa----UGCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 95499 | 0.66 | 0.926299 |
Target: 5'- uGCaggCCGAgaCCa-ACAGGACGUACGaGCu -3' miRNA: 3'- -UGa--GGCUg-GGcaUGUCCUGCAUGC-CG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 30375 | 0.66 | 0.926299 |
Target: 5'- cGCggCGACUCGggUGCGGGcCGgcgcgGCGGCg -3' miRNA: 3'- -UGagGCUGGGC--AUGUCCuGCa----UGCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 26168 | 0.66 | 0.926299 |
Target: 5'- gUUCCG-CCgGcGCGGGcGCGUcgACGGCg -3' miRNA: 3'- uGAGGCuGGgCaUGUCC-UGCA--UGCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 89557 | 0.67 | 0.909515 |
Target: 5'- cGC-CCGAuCCUGUAUAGGuuucgcucGCGguucaGCGGCg -3' miRNA: 3'- -UGaGGCU-GGGCAUGUCC--------UGCa----UGCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 144438 | 0.67 | 0.903463 |
Target: 5'- uACUCCGccgaggacgcGCgCGUGuCGGGcgGCGUGCGGg -3' miRNA: 3'- -UGAGGC----------UGgGCAU-GUCC--UGCAUGCCg -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 28918 | 0.67 | 0.903463 |
Target: 5'- aGCUCCGAgUCGUugGGcuugcacGCGgcugggGCGGCa -3' miRNA: 3'- -UGAGGCUgGGCAugUCc------UGCa-----UGCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 95018 | 0.67 | 0.903463 |
Target: 5'- gGCUUCGugCCGaugUGCGGcGGgG-ACGGCa -3' miRNA: 3'- -UGAGGCugGGC---AUGUC-CUgCaUGCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 158037 | 0.67 | 0.897186 |
Target: 5'- cGCcgCCGcGCCCGUGCguAGcGACGacucgccgaggaUACGGCg -3' miRNA: 3'- -UGa-GGC-UGGGCAUG--UC-CUGC------------AUGCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 146503 | 0.67 | 0.897186 |
Target: 5'- cCUCUGGCCC--GCGGGAuCGUcgGGCa -3' miRNA: 3'- uGAGGCUGGGcaUGUCCU-GCAugCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 56520 | 0.67 | 0.897186 |
Target: 5'- gGCUCgGcgccaaGCUCGgGCGGGGCGggcuCGGCg -3' miRNA: 3'- -UGAGgC------UGGGCaUGUCCUGCau--GCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 56466 | 0.67 | 0.897186 |
Target: 5'- gGCUCgGcgccaaGCUCGgGCGGGGCGggcuCGGCg -3' miRNA: 3'- -UGAGgC------UGGGCaUGUCCUGCau--GCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 14002 | 0.67 | 0.897186 |
Target: 5'- gGCUCuccaaCGACCgGUgGCGGGcgGCGU-CGGCg -3' miRNA: 3'- -UGAG-----GCUGGgCA-UGUCC--UGCAuGCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 55951 | 0.67 | 0.890686 |
Target: 5'- gACUCgGGCCCGUucuguCGGGAgagGCGcGCg -3' miRNA: 3'- -UGAGgCUGGGCAu----GUCCUgcaUGC-CG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 9796 | 0.67 | 0.890686 |
Target: 5'- uGCUCCGGCgUG-ACGGaGGCcacgACGGCg -3' miRNA: 3'- -UGAGGCUGgGCaUGUC-CUGca--UGCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 157880 | 0.67 | 0.883968 |
Target: 5'- cGCUgCGGCUCGUGucGGAgGUcGCGGCc -3' miRNA: 3'- -UGAgGCUGGGCAUguCCUgCA-UGCCG- -5' |
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7647 | 3' | -56.1 | NC_001973.1 | + | 120302 | 0.67 | 0.877034 |
Target: 5'- uAUUCCaaggcgaaugGugUCGUGCGGaGACG-ACGGCg -3' miRNA: 3'- -UGAGG----------CugGGCAUGUC-CUGCaUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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