Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7649 | 3' | -52.8 | NC_001973.1 | + | 40783 | 0.66 | 0.966875 |
Target: 5'- cGUGACAUCAUuCCUUGGagcgCGAucGAAg -3' miRNA: 3'- -UACUGUAGUAcGGAGCCg---GCUuuCUU- -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 151015 | 0.66 | 0.959858 |
Target: 5'- uAUGAUugugccugcuGUCcgG-CUCGGCCGAAGGGu -3' miRNA: 3'- -UACUG----------UAGuaCgGAGCCGGCUUUCUu -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 23082 | 0.67 | 0.951879 |
Target: 5'- gAUGGCG-C-UGCgCUCGGCCG-AAGAGc -3' miRNA: 3'- -UACUGUaGuACG-GAGCCGGCuUUCUU- -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 84327 | 0.67 | 0.947516 |
Target: 5'- -gGACGUCA-GCUaCGGCCGGcugcAGGAGg -3' miRNA: 3'- uaCUGUAGUaCGGaGCCGGCU----UUCUU- -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 72346 | 0.68 | 0.915944 |
Target: 5'- gAUGACAUCAUcCCuaacucgagcUCGGCCGAcgcguAAGAu -3' miRNA: 3'- -UACUGUAGUAcGG----------AGCCGGCU-----UUCUu -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 122634 | 0.69 | 0.889737 |
Target: 5'- -gGGCG-CcgGCgUCGGUCGAGAGAGc -3' miRNA: 3'- uaCUGUaGuaCGgAGCCGGCUUUCUU- -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 2663 | 0.69 | 0.867484 |
Target: 5'- gAUGACAUCAcGCCcgagcucgagcUCGGCCGAc---- -3' miRNA: 3'- -UACUGUAGUaCGG-----------AGCCGGCUuucuu -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 130756 | 0.7 | 0.816967 |
Target: 5'- gAUGACAUCAUGCCcaaacucgagcUCGGCgGGcgcguAGGAu -3' miRNA: 3'- -UACUGUAGUACGG-----------AGCCGgCU-----UUCUu -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 127318 | 0.7 | 0.816967 |
Target: 5'- -cGACAUCAcGCCUCGaGcCCGAuGAGAu -3' miRNA: 3'- uaCUGUAGUaCGGAGC-C-GGCU-UUCUu -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 72190 | 0.7 | 0.816967 |
Target: 5'- aAUGACAUCAUGCugaaguugagCUCGGCgGAcgcguAAGAu -3' miRNA: 3'- -UACUGUAGUACG----------GAGCCGgCU-----UUCUu -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 21042 | 0.7 | 0.816967 |
Target: 5'- gAUGACAUCAUGCCcgagcucgagcUCGGCgGGcgcguAGGAu -3' miRNA: 3'- -UACUGUAGUACGG-----------AGCCGgCU-----UUCUu -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 127494 | 0.71 | 0.807857 |
Target: 5'- gAUGACAUCAUGCCuaaacugagcUCGGCgGGcgcguAGGAu -3' miRNA: 3'- -UACUGUAGUACGG----------AGCCGgCU-----UUCUu -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 16498 | 0.71 | 0.807857 |
Target: 5'- gAUGACAUCAUGCuucgcucgagCUCGGCgGAcgcguAAGAu -3' miRNA: 3'- -UACUGUAGUACG----------GAGCCGgCU-----UUCUu -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 16420 | 0.71 | 0.807857 |
Target: 5'- gAUGACAUCAUGCuucgcucgagCUCGGCgGAcgcguAAGAu -3' miRNA: 3'- -UACUGUAGUACG----------GAGCCGgCU-----UUCUu -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 129040 | 0.71 | 0.779528 |
Target: 5'- gAUGACAUCAUGCCuaaacugagcUCGGuuGAu-GAAu -3' miRNA: 3'- -UACUGUAGUACGG----------AGCCggCUuuCUU- -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 21219 | 0.71 | 0.779528 |
Target: 5'- gAUGACAUCAUGCCgaacUCGGUgGAuuuuacgcgucGAGAu -3' miRNA: 3'- -UACUGUAGUACGG----AGCCGgCU-----------UUCUu -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 130854 | 0.72 | 0.729578 |
Target: 5'- gAUGACAUCAUGCCuaaacucgagcUCGGCgGAcgcguAGGAu -3' miRNA: 3'- -UACUGUAGUACGG-----------AGCCGgCU-----UUCUu -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 129284 | 0.72 | 0.729578 |
Target: 5'- gAUGACAUCAUGCCcaaacucgagcUCGGCgGAcgcguAGGAu -3' miRNA: 3'- -UACUGUAGUACGG-----------AGCCGgCU-----UUCUu -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 12093 | 0.72 | 0.729578 |
Target: 5'- gAUGACAUCAUGCCcaaacucgagcUCGGCgGAcgcguAGGAu -3' miRNA: 3'- -UACUGUAGUACGG-----------AGCCGgCU-----UUCUu -5' |
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7649 | 3' | -52.8 | NC_001973.1 | + | 160446 | 0.72 | 0.719272 |
Target: 5'- gAUGACAUCAUGCCuaaacugagcUCGGCgGAcgcguAGGAu -3' miRNA: 3'- -UACUGUAGUACGG----------AGCCGgCU-----UUCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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