Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7649 | 5' | -56.3 | NC_001973.1 | + | 21017 | 0.66 | 0.898068 |
Target: 5'- aGCCCGau--GAGaucaugcuuguaCCGAUGACAUCAUg -3' miRNA: 3'- aCGGGCcaguCUC------------GGCUACUGUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 14616 | 0.66 | 0.884569 |
Target: 5'- cGCCCGGcugUCGGuGCgCGAcuACGUCAa -3' miRNA: 3'- aCGGGCC---AGUCuCG-GCUacUGUAGUa -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 127463 | 0.66 | 0.88387 |
Target: 5'- gUGCCggcaagGGUCAaucGAGuucaaacCCGAUGACAUCAUg -3' miRNA: 3'- -ACGGg-----CCAGU---CUC-------GGCUACUGUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 130830 | 0.66 | 0.877478 |
Target: 5'- aGCCCGaUgAGAucaugccuacCCGAUGACAUCAUg -3' miRNA: 3'- aCGGGCcAgUCUc---------GGCUACUGUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 12145 | 0.66 | 0.870165 |
Target: 5'- cUGCCgGgGUCAaucgaguucGAGcCCGAUGAgAUCAUg -3' miRNA: 3'- -ACGGgC-CAGU---------CUC-GGCUACUgUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 160420 | 0.66 | 0.861872 |
Target: 5'- gGCCgGuGUCGaucGAGuucaaacCCGAUGACAUCAUg -3' miRNA: 3'- aCGGgC-CAGU---CUC-------GGCUACUGUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 130574 | 0.66 | 0.861872 |
Target: 5'- gGCCgGgGUCGaucGAGuucaaacCCGAUGACAUCAUg -3' miRNA: 3'- aCGGgC-CAGU---CUC-------GGCUACUGUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 72242 | 0.67 | 0.854111 |
Target: 5'- gGCCgGgGUCAaucGAGuucaagcCCGAUGACAUCAUc -3' miRNA: 3'- aCGGgC-CAGU---CUC-------GGCUACUGUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 16571 | 0.67 | 0.854111 |
Target: 5'- aGCCCGaUgAGAucaugccGCCGAUGAgAUCAUu -3' miRNA: 3'- aCGGGCcAgUCU-------CGGCUACUgUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 137873 | 0.67 | 0.83048 |
Target: 5'- aGCgCGGUCAGGGCC----GCGUCGg -3' miRNA: 3'- aCGgGCCAGUCUCGGcuacUGUAGUa -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 21193 | 0.68 | 0.80442 |
Target: 5'- gGCCgGGgucgaUCGGguucaAGcCCGAUGACAUCAUg -3' miRNA: 3'- aCGGgCC-----AGUC-----UC-GGCUACUGUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 16472 | 0.68 | 0.803525 |
Target: 5'- gGCCgGgGUCAaucGAGuucaagcCCGAUGACAUCAUg -3' miRNA: 3'- aCGGgC-CAGU---CUC-------GGCUACUGUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 130725 | 0.68 | 0.789925 |
Target: 5'- cGCgUCGGcCGGGGCaaucgaguucaagacCGAUGACAUCAUg -3' miRNA: 3'- aCG-GGCCaGUCUCG---------------GCUACUGUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 12224 | 0.69 | 0.747305 |
Target: 5'- gGCCagGGUCGaucGAGuucaagcCCGAUGACAUCAUg -3' miRNA: 3'- aCGGg-CCAGU---CUC-------GGCUACUGUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 129009 | 0.69 | 0.732595 |
Target: 5'- cGCgUCGGcCAGGGucgauaaaggucaaaCCGAUGACAUCAUg -3' miRNA: 3'- aCG-GGCCaGUCUC---------------GGCUACUGUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 158441 | 0.69 | 0.70866 |
Target: 5'- cGCCCGcGUCcGAGCCGGcu-CGUCAa -3' miRNA: 3'- aCGGGC-CAGuCUCGGCUacuGUAGUa -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 72081 | 0.69 | 0.70866 |
Target: 5'- aGCCCGaugacaUCAugcuGCCGGUGACAUCAUc -3' miRNA: 3'- aCGGGCc-----AGUcu--CGGCUACUGUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 150872 | 0.7 | 0.698568 |
Target: 5'- gGCCUGGcCGGAGCCGG-GGCG-CGUu -3' miRNA: 3'- aCGGGCCaGUCUCGGCUaCUGUaGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 72316 | 0.7 | 0.697556 |
Target: 5'- cGCCCgcuagGGUCaacAGAGuucaagcCCGAUGACAUCAUc -3' miRNA: 3'- aCGGG-----CCAG---UCUC-------GGCUACUGUAGUA- -5' |
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7649 | 5' | -56.3 | NC_001973.1 | + | 129266 | 0.7 | 0.66799 |
Target: 5'- gGUCaucgGGUUcaAGCCGAUGACAUCAUg -3' miRNA: 3'- aCGGg---CCAGucUCGGCUACUGUAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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