miRNA: 3'- aCGGg---CCAGucUCGGCUACUGUAGUA- -5'
miRNA: 3'- aCGggC-CAGUCUcGGCUACUGUAGUa -5'
miRNA: 3'- -ACG-GGCCaGUCUCGGCUACUgUAGUA- -5'
miRNA: 3'- aCGGGCCaGUCUCGGCUACUGUAGUA- -5'
miRNA: 3'- -ACGGGCCAGUCUCGGCUACUGUAGUA- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7649 | 5' | -56.3 | NC_001973.1 | + | 129266 | 0.7 | 0.66799 |
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Target: 5'- gGUCaucgGGUUcaAGCCGAUGACAUCAUg -3' miRNA: 3'- aCGGg---CCAGucUCGGCUACUGUAGUA- -5' |
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| 7649 | 5' | -56.3 | NC_001973.1 | + | 129579 | 0.71 | 0.606264 |
|
Target: 5'- cGCaaGcGUCAGGcCCGAUGACAUCAg -3' miRNA: 3'- aCGggC-CAGUCUcGGCUACUGUAGUa -5' |
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| 7649 | 5' | -56.3 | NC_001973.1 | + | 128945 | 0.74 | 0.439572 |
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Target: 5'- aUGCgCCGGcUAGGGUCGAUGAgAUCAUg -3' miRNA: 3'- -ACG-GGCCaGUCUCGGCUACUgUAGUA- -5' |
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| 7649 | 5' | -56.3 | NC_001973.1 | + | 151133 | 0.93 | 0.030146 |
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Target: 5'- cGCUCGGcCAGAGCCGAUGACAUCAUg -3' miRNA: 3'- aCGGGCCaGUCUCGGCUACUGUAGUA- -5' |
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| 7649 | 5' | -56.3 | NC_001973.1 | + | 151182 | 1.06 | 0.003847 |
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Target: 5'- cUGCCCGGUCAGAGCCGAUGACAUCAUg -3' miRNA: 3'- -ACGGGCCAGUCUCGGCUACUGUAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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