miRNA: 3'- aCGGgC-CAGU---CUC-------GGCUACUGUAGUA- -5'
miRNA: 3'- aCGGGCcAgUCU-------CGGCUACUgUAGUA- -5'
miRNA: 3'- aCGgGCCAGUCUCGGcuacUGUAGUa -5'
miRNA: 3'- aCGGgCC-----AGUC-----UC-GGCUACUGUAGUA- -5'
miRNA: 3'- -ACGGGCCAGUCUCGGCUACUGUAGUA- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7649 | 5' | -56.3 | NC_001973.1 | + | 72242 | 0.67 | 0.854111 |
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Target: 5'- gGCCgGgGUCAaucGAGuucaagcCCGAUGACAUCAUc -3' miRNA: 3'- aCGGgC-CAGU---CUC-------GGCUACUGUAGUA- -5' |
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| 7649 | 5' | -56.3 | NC_001973.1 | + | 16571 | 0.67 | 0.854111 |
|
Target: 5'- aGCCCGaUgAGAucaugccGCCGAUGAgAUCAUu -3' miRNA: 3'- aCGGGCcAgUCU-------CGGCUACUgUAGUA- -5' |
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| 7649 | 5' | -56.3 | NC_001973.1 | + | 137873 | 0.67 | 0.83048 |
|
Target: 5'- aGCgCGGUCAGGGCC----GCGUCGg -3' miRNA: 3'- aCGgGCCAGUCUCGGcuacUGUAGUa -5' |
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| 7649 | 5' | -56.3 | NC_001973.1 | + | 21193 | 0.68 | 0.80442 |
|
Target: 5'- gGCCgGGgucgaUCGGguucaAGcCCGAUGACAUCAUg -3' miRNA: 3'- aCGGgCC-----AGUC-----UC-GGCUACUGUAGUA- -5' |
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| 7649 | 5' | -56.3 | NC_001973.1 | + | 151182 | 1.06 | 0.003847 |
|
Target: 5'- cUGCCCGGUCAGAGCCGAUGACAUCAUg -3' miRNA: 3'- -ACGGGCCAGUCUCGGCUACUGUAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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