Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7659 | 3' | -52.7 | NC_001973.1 | + | 139036 | 0.7 | 0.862738 |
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Target: 5'- aUGAACGCGCuGCCgcgGAAGuCGGCgaaGUGc -3' miRNA: 3'- gACUUGUGCG-UGGa--CUUC-GUCGa--CAC- -5' |
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| 7659 | 3' | -52.7 | NC_001973.1 | + | 139844 | 0.66 | 0.967523 |
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Target: 5'- --cGACGCGCACCUGuccGCGGaccgGUGc -3' miRNA: 3'- gacUUGUGCGUGGACuu-CGUCga--CAC- -5' |
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| 7659 | 3' | -52.7 | NC_001973.1 | + | 140366 | 0.68 | 0.934367 |
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Target: 5'- aUGAGCGuCGuCGCCgccGAGCGGCUGg- -3' miRNA: 3'- gACUUGU-GC-GUGGac-UUCGUCGACac -5' |
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| 7659 | 3' | -52.7 | NC_001973.1 | + | 143002 | 1.08 | 0.005815 |
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Target: 5'- aCUGAACACGCACCUGAAGCAGCUGUGc -3' miRNA: 3'- -GACUUGUGCGUGGACUUCGUCGACAC- -5' |
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| 7659 | 3' | -52.7 | NC_001973.1 | + | 144448 | 0.7 | 0.870455 |
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Target: 5'- -aGGACGCGCGCgUGucgGGCGGC-GUGc -3' miRNA: 3'- gaCUUGUGCGUGgACu--UCGUCGaCAC- -5' |
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| 7659 | 3' | -52.7 | NC_001973.1 | + | 146666 | 0.67 | 0.944129 |
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Target: 5'- uUGAGCGCGCucacgagguCCUGGAcCAGC-GUGg -3' miRNA: 3'- gACUUGUGCGu--------GGACUUcGUCGaCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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