miRNA: 3'- -CUAGUGcuacuCGUGCUACGGGUGGU- -5'
miRNA: 3'- cuagUGCUaCUCGUGCUaCGGGUGGU- -5'
miRNA: 3'- cuAGU---GCUACUCGUGCua---CGGGUGGU- -5'
miRNA: 3'- cuaGUGCUACUCGUgCu---ACGGGUGGU- -5'
miRNA: 3'- -CUAGUGCUACUCGUGCUACGGGUGGU- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7670 | 3' | -54 | NC_001973.1 | + | 128174 | 0.75 | 0.527818 |
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Target: 5'- uGAUCACauccuGCGCGAUGUCCGCCAu -3' miRNA: 3'- -CUAGUGcuacuCGUGCUACGGGUGGU- -5' |
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| 7670 | 3' | -54 | NC_001973.1 | + | 573 | 0.76 | 0.4787 |
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Target: 5'- ---aGCGAcGGGCACGA-GCCCGCCAc -3' miRNA: 3'- cuagUGCUaCUCGUGCUaCGGGUGGU- -5' |
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| 7670 | 3' | -54 | NC_001973.1 | + | 21478 | 0.77 | 0.450328 |
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Target: 5'- -cUCAccuCGGUGAGCGCGucgcgGCCCGCCGa -3' miRNA: 3'- cuAGU---GCUACUCGUGCua---CGGGUGGU- -5' |
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| 7670 | 3' | -54 | NC_001973.1 | + | 74647 | 0.79 | 0.354948 |
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Target: 5'- --gCGCGGUGAGCGgGuaguUGCCCACCGg -3' miRNA: 3'- cuaGUGCUACUCGUgCu---ACGGGUGGU- -5' |
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| 7670 | 3' | -54 | NC_001973.1 | + | 132949 | 1.08 | 0.005011 |
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Target: 5'- uGAUCACGAUGAGCACGAUGCCCACCAc -3' miRNA: 3'- -CUAGUGCUACUCGUGCUACGGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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