Results 21 - 39 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8069 | 5' | -53.4 | NC_001973.1 | + | 73613 | 0.71 | 0.828728 |
Target: 5'- gGGCGAUCAGUUCGgacggcucgccgucCGCGCgCucGUCGc -3' miRNA: 3'- -CCGCUAGUUAAGCa-------------GCGCG-GucCAGC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 79621 | 0.7 | 0.868408 |
Target: 5'- uGUGAUaguugacgggCGUCGuCGCCGGGUCGu -3' miRNA: 3'- cCGCUAguuaa-----GCAGC-GCGGUCCAGC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 80043 | 0.68 | 0.948262 |
Target: 5'- uGGUGAUgaCGGgcUUG-CGCGCCGGGUCc -3' miRNA: 3'- -CCGCUA--GUUa-AGCaGCGCGGUCCAGc -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 81077 | 0.67 | 0.956332 |
Target: 5'- gGGacaGGUCGAUggCGuUCGCGCUGGGcgCGg -3' miRNA: 3'- -CCg--CUAGUUAa-GC-AGCGCGGUCCa-GC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 81629 | 0.71 | 0.849977 |
Target: 5'- gGGCGAUCuuggUCGcgCGCGCCGcuUCGg -3' miRNA: 3'- -CCGCUAGuua-AGCa-GCGCGGUccAGC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 81771 | 0.68 | 0.943879 |
Target: 5'- cGCGAUCA---UGUCGCGCUcGGcgCGa -3' miRNA: 3'- cCGCUAGUuaaGCAGCGCGGuCCa-GC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 82177 | 0.67 | 0.964828 |
Target: 5'- cGGCGAgauagccgucgaggCAGUUCuuGUCGCGCUuuucGUCGg -3' miRNA: 3'- -CCGCUa-------------GUUAAG--CAGCGCGGuc--CAGC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 83034 | 0.69 | 0.918402 |
Target: 5'- cGGCGggCGAggCGgCGCGCCc-GUCGa -3' miRNA: 3'- -CCGCuaGUUaaGCaGCGCGGucCAGC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 100283 | 0.69 | 0.899615 |
Target: 5'- gGGCGcgCAAUUCGUuuuugaaCGcCGCCucGUCGg -3' miRNA: 3'- -CCGCuaGUUAAGCA-------GC-GCGGucCAGC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 102641 | 0.67 | 0.952412 |
Target: 5'- cGGCGcgCGAcuggcaaacgCGcUCGUGCCAGGUgCGc -3' miRNA: 3'- -CCGCuaGUUaa--------GC-AGCGCGGUCCA-GC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 102712 | 0.66 | 0.980006 |
Target: 5'- gGGcCGGUCAAgauuUUCGUgGcCGCCA-GUCGc -3' miRNA: 3'- -CC-GCUAGUU----AAGCAgC-GCGGUcCAGC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 122691 | 0.71 | 0.841956 |
Target: 5'- cGGCGAUcCAAUcgcagcagUCGUucgacCGCGCCGucGGUCGc -3' miRNA: 3'- -CCGCUA-GUUA--------AGCA-----GCGCGGU--CCAGC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 126587 | 0.68 | 0.939261 |
Target: 5'- uGGCGAUCAcgUCcugggcgauGUCG-GCCAugcGGUUGg -3' miRNA: 3'- -CCGCUAGUuaAG---------CAGCgCGGU---CCAGC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 126690 | 0.67 | 0.966757 |
Target: 5'- aGCcGUUucggCGUCGCGCCGGGcCa -3' miRNA: 3'- cCGcUAGuuaaGCAGCGCGGUCCaGc -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 134976 | 0.68 | 0.939261 |
Target: 5'- cGGCGAUCGAguaCGUCcgggcCGUCGGG-CGg -3' miRNA: 3'- -CCGCUAGUUaa-GCAGc----GCGGUCCaGC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 136316 | 0.66 | 0.982097 |
Target: 5'- gGGUaGUCGAgcUCGUUGCGCUGGcUCGg -3' miRNA: 3'- -CCGcUAGUUa-AGCAGCGCGGUCcAGC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 136999 | 0.66 | 0.972644 |
Target: 5'- aGCcccUCGAgcgCGU-GCGCCGGGUCGc -3' miRNA: 3'- cCGcu-AGUUaa-GCAgCGCGGUCCAGC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 137089 | 0.7 | 0.872828 |
Target: 5'- uGGUGG-CAGUUCaUCGCGaaCAGGUCGu -3' miRNA: 3'- -CCGCUaGUUAAGcAGCGCg-GUCCAGC- -5' |
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8069 | 5' | -53.4 | NC_001973.1 | + | 150061 | 0.66 | 0.977739 |
Target: 5'- aGGCuggaaaucugGGUCGAUUUcaaaaaucaaGUCGCGCC-GGUCc -3' miRNA: 3'- -CCG----------CUAGUUAAG----------CAGCGCGGuCCAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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