Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
8072 | 5' | -55.9 | NC_001978.2 | + | 39867 | 0.66 | 0.60206 |
Target: 5'- gCUCUUCaagggCGUUCagggcgucgGCGCGCUcCGCAa -3' miRNA: 3'- -GAGAAGg----GCAAGaa-------CGCGCGGuGCGU- -5' |
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8072 | 5' | -55.9 | NC_001978.2 | + | 5777 | 0.67 | 0.514089 |
Target: 5'- -aCUUCgCGUUCUUGCGguaCGCCgACGgGa -3' miRNA: 3'- gaGAAGgGCAAGAACGC---GCGG-UGCgU- -5' |
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8072 | 5' | -55.9 | NC_001978.2 | + | 27240 | 0.68 | 0.45168 |
Target: 5'- uUCUUCCCGg----GCGUGCUcgACGCu -3' miRNA: 3'- gAGAAGGGCaagaaCGCGCGG--UGCGu -5' |
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8072 | 5' | -55.9 | NC_001978.2 | + | 5052 | 0.71 | 0.33236 |
Target: 5'- gCUCUUCCU--UCUcGCGCGCCucaGCGg -3' miRNA: 3'- -GAGAAGGGcaAGAaCGCGCGGug-CGU- -5' |
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8072 | 5' | -55.9 | NC_001978.2 | + | 7061 | 0.72 | 0.288586 |
Target: 5'- gUCUUCCCGcccgaugccaaugCUUGCGUcgaguGCCAUGCAa -3' miRNA: 3'- gAGAAGGGCaa-----------GAACGCG-----CGGUGCGU- -5' |
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8072 | 5' | -55.9 | NC_001978.2 | + | 1587 | 0.97 | 0.004527 |
Target: 5'- gCUCUUCCCGUUCUUG-GCGCCACGCAc -3' miRNA: 3'- -GAGAAGGGCAAGAACgCGCGGUGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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