Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8078 | 3' | -55.8 | NC_001978.2 | + | 33262 | 0.66 | 0.624875 |
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Target: 5'- aGUUGGGcgcuAcgGCGUGAag-GUGGUCGAg -3' miRNA: 3'- -UAGCCC----UuaCGCGCUgugCACCAGCU- -5' |
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| 8078 | 3' | -55.8 | NC_001978.2 | + | 15550 | 0.67 | 0.558234 |
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Target: 5'- aGUCGGGcggcagGgGCGACGuCGgcaaGGUCGAc -3' miRNA: 3'- -UAGCCCuua---CgCGCUGU-GCa---CCAGCU- -5' |
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| 8078 | 3' | -55.8 | NC_001978.2 | + | 4494 | 0.67 | 0.547292 |
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Target: 5'- --aGGGAAcGCGUGu--CGUGGUUGAa -3' miRNA: 3'- uagCCCUUaCGCGCuguGCACCAGCU- -5' |
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| 8078 | 3' | -55.8 | NC_001978.2 | + | 4663 | 0.67 | 0.547292 |
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Target: 5'- uUCGGGAGauCaCGGCuauggACGUGGUCGAg -3' miRNA: 3'- uAGCCCUUacGcGCUG-----UGCACCAGCU- -5' |
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| 8078 | 3' | -55.8 | NC_001978.2 | + | 15734 | 0.68 | 0.50428 |
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Target: 5'- uGUgGGcGAAgucGgGCGGCGUGUGGUCGAa -3' miRNA: 3'- -UAgCC-CUUa--CgCGCUGUGCACCAGCU- -5' |
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| 8078 | 3' | -55.8 | NC_001978.2 | + | 34432 | 0.69 | 0.442734 |
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Target: 5'- -cCGGGGcucagaaaGUGUGCGAa--GUGGUCGGc -3' miRNA: 3'- uaGCCCU--------UACGCGCUgugCACCAGCU- -5' |
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| 8078 | 3' | -55.8 | NC_001978.2 | + | 1371 | 0.71 | 0.309648 |
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Target: 5'- -gCGGGAAUGUGCcgcgcaauCACGgGGUCGAu -3' miRNA: 3'- uaGCCCUUACGCGcu------GUGCaCCAGCU- -5' |
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| 8078 | 3' | -55.8 | NC_001978.2 | + | 12157 | 0.71 | 0.309648 |
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Target: 5'- uAUCGGGGGcacggcuggGgGCGGCACGUgggcauGGUCGAg -3' miRNA: 3'- -UAGCCCUUa--------CgCGCUGUGCA------CCAGCU- -5' |
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| 8078 | 3' | -55.8 | NC_001978.2 | + | 18209 | 0.73 | 0.258818 |
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Target: 5'- uUCGGGAccuucaGCGCGuccCACGUGGUCu- -3' miRNA: 3'- uAGCCCUua----CGCGCu--GUGCACCAGcu -5' |
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| 8078 | 3' | -55.8 | NC_001978.2 | + | 6410 | 1.07 | 0.000846 |
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Target: 5'- gAUCGGGAAUGCGCGACACGUGGUCGAg -3' miRNA: 3'- -UAGCCCUUACGCGCUGUGCACCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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