miRNA: 3'- uUCCAGCcGC------AAGUGCCGU-CGCGUUC- -5'
miRNA: 3'- uUC-CAGCCGCaaGUG---------CCGUCGCGUuc -5'
miRNA: 3'- -UUC-CAGCCGCAAGU-GCc-GUCgCGUUC- -5'
miRNA: 3'- uUCCAGCCGCaaGU---------GCCGUCgCGUuc -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8080 | 3' | -57.2 | NC_001978.2 | + | 18469 | 0.66 | 0.49286 |
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Target: 5'- cAGGUUGcCGacucccUUCACGGCAuGCGUAAGc -3' miRNA: 3'- uUCCAGCcGC------AAGUGCCGU-CGCGUUC- -5' |
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| 8080 | 3' | -57.2 | NC_001978.2 | + | 27109 | 0.66 | 0.49286 |
|
Target: 5'- cGGaGUCGGCGggCAUuguggccugGGCGGUGCGc- -3' miRNA: 3'- uUC-CAGCCGCaaGUG---------CCGUCGCGUuc -5' |
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| 8080 | 3' | -57.2 | NC_001978.2 | + | 8955 | 0.66 | 0.503426 |
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Target: 5'- gAAGuGUCGGCGUUCGgCGaaCAG-GCGAGg -3' miRNA: 3'- -UUC-CAGCCGCAAGU-GCc-GUCgCGUUC- -5' |
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| 8080 | 3' | -57.2 | NC_001978.2 | + | 5944 | 0.66 | 0.514089 |
|
Target: 5'- cAGGUCGGCGaaCAgaaccuuguCGGCAG-GCAu- -3' miRNA: 3'- uUCCAGCCGCaaGU---------GCCGUCgCGUuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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