miRNA: 3'- aaGGUu-CUCAGGCgGACGGGG--AGCAU- -5'
miRNA: 3'- aAGGUu---CUCAG--GCGGACGGgGAGCau -5'
miRNA: 3'- aaGGUUCu-CAGGC---------GGACGGGGAGCau -5'
miRNA: 3'- -AAGGuu---CUCAgGCGGACGGGGAGCau -5'
miRNA: 3'- -AAGGUUCUCAGGCGGACGGGGAGCAU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8081 | 5' | -59.3 | NC_001978.2 | + | 193 | 0.69 | 0.235469 |
|
Target: 5'- -gCCGcuGAGUCCG-CUGCCCCcgUCGUGu -3' miRNA: 3'- aaGGUu-CUCAGGCgGACGGGG--AGCAU- -5' |
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| 8081 | 5' | -59.3 | NC_001978.2 | + | 29145 | 0.68 | 0.268395 |
|
Target: 5'- gUCCGucacGGGUCggCGCCUGCCgCUCGa- -3' miRNA: 3'- aAGGUu---CUCAG--GCGGACGGgGAGCau -5' |
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| 8081 | 5' | -59.3 | NC_001978.2 | + | 11712 | 0.66 | 0.345178 |
|
Target: 5'- -aCCGGGucGUCCGgcgccggucCCUGCCCUUCGa- -3' miRNA: 3'- aaGGUUCu-CAGGC---------GGACGGGGAGCau -5' |
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| 8081 | 5' | -59.3 | NC_001978.2 | + | 30413 | 0.67 | 0.297334 |
|
Target: 5'- gUUCCuugcuGAGU-CGCUUGCCCUUCGg- -3' miRNA: 3'- -AAGGuu---CUCAgGCGGACGGGGAGCau -5' |
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| 8081 | 5' | -59.3 | NC_001978.2 | + | 7121 | 1.04 | 0.000485 |
|
Target: 5'- cUUCCAAGAGUCCGCCUGCCCCUCGUAa -3' miRNA: 3'- -AAGGUUCUCAGGCGGACGGGGAGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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