Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8082 | 5' | -53.3 | NC_001978.2 | + | 13286 | 0.66 | 0.750296 |
Target: 5'- gCGGCaaggUCGGCCGUCGuGG-UCCAc-- -3' miRNA: 3'- aGUUGa---AGCCGGCAGU-CCgAGGUaag -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 20403 | 0.66 | 0.716268 |
Target: 5'- gUCAGCUccgucucUCGcGCCGUgCGGGCUUCcUUg -3' miRNA: 3'- -AGUUGA-------AGC-CGGCA-GUCCGAGGuAAg -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 18764 | 0.66 | 0.70621 |
Target: 5'- cCGAaucCUUCaGCaCGUCGGGCagaCCGUUCg -3' miRNA: 3'- aGUU---GAAGcCG-GCAGUCCGa--GGUAAG- -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 10941 | 0.67 | 0.683644 |
Target: 5'- cUCGACggucCGGCCGaaccaugcguugUCGGGUUCCGc-- -3' miRNA: 3'- -AGUUGaa--GCCGGC------------AGUCCGAGGUaag -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 8192 | 0.67 | 0.683644 |
Target: 5'- cUCGACUUCGGgucgcggugaCCGUCcGGGCUUUcgUg -3' miRNA: 3'- -AGUUGAAGCC----------GGCAG-UCCGAGGuaAg -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 1477 | 0.67 | 0.660865 |
Target: 5'- aCAcCUUCGGCCGUU-GGCgCCGg-- -3' miRNA: 3'- aGUuGAAGCCGGCAGuCCGaGGUaag -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 36843 | 0.68 | 0.61505 |
Target: 5'- -aGGCUcCGGC--UCAGGCUCCGgcgUCg -3' miRNA: 3'- agUUGAaGCCGgcAGUCCGAGGUa--AG- -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 20446 | 0.68 | 0.602466 |
Target: 5'- cUCGACUUCGGCCuUCGccgcuucGGCUuCCGg-- -3' miRNA: 3'- -AGUUGAAGCCGGcAGU-------CCGA-GGUaag -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 12863 | 0.68 | 0.580817 |
Target: 5'- aUCGcGCUUauGUCGUCGGGCUCgAUUg -3' miRNA: 3'- -AGU-UGAAgcCGGCAGUCCGAGgUAAg -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 31378 | 0.69 | 0.535858 |
Target: 5'- -gGGCUUCGGCCcau-GGCUCCGg-- -3' miRNA: 3'- agUUGAAGCCGGcaguCCGAGGUaag -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 25057 | 0.7 | 0.502951 |
Target: 5'- -gGGCUUCGGaCCGaaggUCAGcGC-CCAUUCg -3' miRNA: 3'- agUUGAAGCC-GGC----AGUC-CGaGGUAAG- -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 21179 | 0.7 | 0.502951 |
Target: 5'- uUCAGCgcgUCGGCCGUCuucucGGCgucgCUcUUCu -3' miRNA: 3'- -AGUUGa--AGCCGGCAGu----CCGa---GGuAAG- -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 16576 | 0.71 | 0.410285 |
Target: 5'- uUCggUUUaCGGUCGUC-GGCUCCGUg- -3' miRNA: 3'- -AGuuGAA-GCCGGCAGuCCGAGGUAag -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 30560 | 0.71 | 0.410285 |
Target: 5'- cCGAcCUUCGggcgaaucucGCCGUCGGGCUCUucgUCg -3' miRNA: 3'- aGUU-GAAGC----------CGGCAGUCCGAGGua-AG- -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 26119 | 0.72 | 0.400653 |
Target: 5'- gCGACUUCGGCCuuaCGGuGCUCCGccUCa -3' miRNA: 3'- aGUUGAAGCCGGca-GUC-CGAGGUa-AG- -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 28621 | 0.73 | 0.319908 |
Target: 5'- aCGGCUUCGGCCaacggGUUAGGCUCacgcgccccgauaCGUUCc -3' miRNA: 3'- aGUUGAAGCCGG-----CAGUCCGAG-------------GUAAG- -5' |
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8082 | 5' | -53.3 | NC_001978.2 | + | 7454 | 1.11 | 0.000737 |
Target: 5'- cUCAACUUCGGCCGUCAGGCUCCAUUCg -3' miRNA: 3'- -AGUUGAAGCCGGCAGUCCGAGGUAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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