Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 8086 | 3' | -58 | NC_001978.2 | + | 5656 | 0.69 | 0.323435 |
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Target: 5'- gAUGCCGCGCGgcugaccCGUgCCGguACC-CGUCAg -3' miRNA: 3'- gUACGGCGCGU-------GCA-GGC--UGGuGCAGU- -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 37553 | 0.69 | 0.340338 |
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Target: 5'- --aGCUucauGCGCaACGggCCGACCACGUUg -3' miRNA: 3'- guaCGG----CGCG-UGCa-GGCUGGUGCAGu -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 39494 | 0.69 | 0.348609 |
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Target: 5'- -uUGCCGCGCccccugcCGUCCaACCACGcCu -3' miRNA: 3'- guACGGCGCGu------GCAGGcUGGUGCaGu -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 22271 | 0.72 | 0.227352 |
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Target: 5'- --aGCCGUaCGUGUCCGACCACGcCAc -3' miRNA: 3'- guaCGGCGcGUGCAGGCUGGUGCaGU- -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 12411 | 0.74 | 0.147783 |
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Target: 5'- --cGCCGCGU-UGUCCGGCCGUGUCGg -3' miRNA: 3'- guaCGGCGCGuGCAGGCUGGUGCAGU- -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 14228 | 0.77 | 0.091741 |
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Target: 5'- cCAUGCCGCGCcCGauagCCGACCAC-UCGa -3' miRNA: 3'- -GUACGGCGCGuGCa---GGCUGGUGcAGU- -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 8782 | 1.08 | 0.000458 |
|
Target: 5'- cCAUGCCGCGCACGUCCGACCACGUCAa -3' miRNA: 3'- -GUACGGCGCGUGCAGGCUGGUGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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