Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8086 | 3' | -58 | NC_001978.2 | + | 26532 | 0.66 | 0.488817 |
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Target: 5'- -uUGCC-CGUACGaggUCGACCACGcCGu -3' miRNA: 3'- guACGGcGCGUGCa--GGCUGGUGCaGU- -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 20536 | 0.66 | 0.499115 |
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Target: 5'- --aGuuGgCGCACGUCaugcuGACCauGCGUCAg -3' miRNA: 3'- guaCggC-GCGUGCAGg----CUGG--UGCAGU- -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 26676 | 0.66 | 0.509509 |
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Target: 5'- --aGCCGaCGCAUGUagCCGACaAgGUCAg -3' miRNA: 3'- guaCGGC-GCGUGCA--GGCUGgUgCAGU- -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 14575 | 0.66 | 0.509509 |
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Target: 5'- gGUGaUUGUGCGCGUCCGGCguCGg-- -3' miRNA: 3'- gUAC-GGCGCGUGCAGGCUGguGCagu -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 6805 | 0.66 | 0.509509 |
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Target: 5'- -cUGCCGUGaACG-CCGACCuugUGUCGg -3' miRNA: 3'- guACGGCGCgUGCaGGCUGGu--GCAGU- -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 12388 | 0.66 | 0.509509 |
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Target: 5'- uGUGCCGaagGCGU-CGACCACGUUc -3' miRNA: 3'- gUACGGCgcgUGCAgGCUGGUGCAGu -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 29172 | 0.66 | 0.519993 |
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Target: 5'- cCGUGCCGCGCGCuUCaCGcuGCCaACG-CGa -3' miRNA: 3'- -GUACGGCGCGUGcAG-GC--UGG-UGCaGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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