Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8092 | 5' | -54.9 | NC_001978.2 | + | 36349 | 0.66 | 0.604556 |
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Target: 5'- cGACGcccuUCGUCAGCgCGCaCAGCGAa--- -3' miRNA: 3'- -CUGC----AGCAGUCG-GCGcGUUGCUaguu -5' |
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| 8092 | 5' | -54.9 | NC_001978.2 | + | 23987 | 0.66 | 0.627258 |
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Target: 5'- aGCGUCGagcacgcucacUCAGCacaGCGCGACGggCu- -3' miRNA: 3'- cUGCAGC-----------AGUCGg--CGCGUUGCuaGuu -5' |
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| 8092 | 5' | -54.9 | NC_001978.2 | + | 25512 | 0.66 | 0.627258 |
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Target: 5'- cGACGUCGUUcGCCcgguucccGCGC-AUGGUCGu -3' miRNA: 3'- -CUGCAGCAGuCGG--------CGCGuUGCUAGUu -5' |
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| 8092 | 5' | -54.9 | NC_001978.2 | + | 20471 | 0.66 | 0.638621 |
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Target: 5'- cGGCGaCGUCGGCCgaccgGCGCGAC--UCGAc -3' miRNA: 3'- -CUGCaGCAGUCGG-----CGCGUUGcuAGUU- -5' |
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| 8092 | 5' | -54.9 | NC_001978.2 | + | 40222 | 0.66 | 0.649978 |
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Target: 5'- -uCGUCGUCGGUCG-GCGGCuucgcccacguGAUCGAa -3' miRNA: 3'- cuGCAGCAGUCGGCgCGUUG-----------CUAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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