Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8092 | 5' | -54.9 | NC_001978.2 | + | 10962 | 0.71 | 0.323392 |
Target: 5'- uGCGUUGUCGgguuccGCgGCGUGACGGUCAAc -3' miRNA: 3'- cUGCAGCAGU------CGgCGCGUUGCUAGUU- -5' |
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8092 | 5' | -54.9 | NC_001978.2 | + | 31147 | 0.72 | 0.30737 |
Target: 5'- cGGCGuUCGUCAGUCGCucagcgGCAGCGGcuUCGAg -3' miRNA: 3'- -CUGC-AGCAGUCGGCG------CGUUGCU--AGUU- -5' |
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8092 | 5' | -54.9 | NC_001978.2 | + | 33937 | 0.72 | 0.299589 |
Target: 5'- -uCGUCGUCGGCguacaCGCGC-ACGGUCGg -3' miRNA: 3'- cuGCAGCAGUCG-----GCGCGuUGCUAGUu -5' |
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8092 | 5' | -54.9 | NC_001978.2 | + | 13455 | 0.73 | 0.26297 |
Target: 5'- cGACGUCGUCAGCCGCGaagu-GUCc- -3' miRNA: 3'- -CUGCAGCAGUCGGCGCguugcUAGuu -5' |
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8092 | 5' | -54.9 | NC_001978.2 | + | 12318 | 1.06 | 0.001037 |
Target: 5'- cGACGUCGUCAGCCGCGCAACGAUCAAg -3' miRNA: 3'- -CUGCAGCAGUCGGCGCGUUGCUAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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