Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8101 | 3' | -61 | NC_001978.2 | + | 15999 | 0.66 | 0.34512 |
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Target: 5'- gCGCCCGuCGCGCCcguGUCGcccuugucgcccuucGCGcCCUUGa -3' miRNA: 3'- -GCGGGCcGUGCGG---CAGU---------------CGCaGGAAC- -5' |
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| 8101 | 3' | -61 | NC_001978.2 | + | 5847 | 0.66 | 0.341879 |
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Target: 5'- uGCCacaGGUACuGaCCGUUcGCGUCCUUc -3' miRNA: 3'- gCGGg--CCGUG-C-GGCAGuCGCAGGAAc -5' |
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| 8101 | 3' | -61 | NC_001978.2 | + | 17466 | 0.66 | 0.341879 |
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Target: 5'- cCGCCgacCGuGUACGCauaGUCAGCGUCgUa- -3' miRNA: 3'- -GCGG---GC-CGUGCGg--CAGUCGCAGgAac -5' |
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| 8101 | 3' | -61 | NC_001978.2 | + | 11087 | 0.66 | 0.341072 |
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Target: 5'- gCGCCCaGGCgacuucGCGCCcaaucgcgaacggGUCGGCGUUCg-- -3' miRNA: 3'- -GCGGG-CCG------UGCGG-------------CAGUCGCAGGaac -5' |
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| 8101 | 3' | -61 | NC_001978.2 | + | 38694 | 0.67 | 0.326006 |
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Target: 5'- cCGCCCGGCGCGgcauUCGUUcaggauGCGUUCg-- -3' miRNA: 3'- -GCGGGCCGUGC----GGCAGu-----CGCAGGaac -5' |
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| 8101 | 3' | -61 | NC_001978.2 | + | 30513 | 0.67 | 0.310686 |
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Target: 5'- aGCgugUCGGCAagggaaCCGUCGGCGUCCg-- -3' miRNA: 3'- gCG---GGCCGUgc----GGCAGUCGCAGGaac -5' |
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| 8101 | 3' | -61 | NC_001978.2 | + | 1825 | 0.68 | 0.281705 |
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Target: 5'- aGCCCuGU-UGCCGUcCGGCGUCCg-- -3' miRNA: 3'- gCGGGcCGuGCGGCA-GUCGCAGGaac -5' |
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| 8101 | 3' | -61 | NC_001978.2 | + | 17912 | 1.07 | 0.000299 |
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Target: 5'- uCGCCCGGCACGCCGUCAGCGUCCUUGc -3' miRNA: 3'- -GCGGGCCGUGCGGCAGUCGCAGGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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