Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8101 | 3' | -61 | NC_001978.2 | + | 17466 | 0.66 | 0.341879 |
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Target: 5'- cCGCCgacCGuGUACGCauaGUCAGCGUCgUa- -3' miRNA: 3'- -GCGG---GC-CGUGCGg--CAGUCGCAGgAac -5' |
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| 8101 | 3' | -61 | NC_001978.2 | + | 5847 | 0.66 | 0.341879 |
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Target: 5'- uGCCacaGGUACuGaCCGUUcGCGUCCUUc -3' miRNA: 3'- gCGGg--CCGUG-C-GGCAGuCGCAGGAAc -5' |
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| 8101 | 3' | -61 | NC_001978.2 | + | 15999 | 0.66 | 0.34512 |
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Target: 5'- gCGCCCGuCGCGCCcguGUCGcccuugucgcccuucGCGcCCUUGa -3' miRNA: 3'- -GCGGGCcGUGCGG---CAGU---------------CGCaGGAAC- -5' |
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| 8101 | 3' | -61 | NC_001978.2 | + | 34307 | 0.66 | 0.361654 |
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Target: 5'- uGCCCGGCAUGuuGgacguugauacgggCAGCGgguaUCCg-- -3' miRNA: 3'- gCGGGCCGUGCggCa-------------GUCGC----AGGaac -5' |
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| 8101 | 3' | -61 | NC_001978.2 | + | 15320 | 0.66 | 0.36672 |
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Target: 5'- aCGCCaaCGGCAa--CGUCGGCGUUCg-- -3' miRNA: 3'- -GCGG--GCCGUgcgGCAGUCGCAGGaac -5' |
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| 8101 | 3' | -61 | NC_001978.2 | + | 20504 | 0.66 | 0.375272 |
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Target: 5'- gGUCgGGCACGCCuUCAaUGUCgUUGa -3' miRNA: 3'- gCGGgCCGUGCGGcAGUcGCAGgAAC- -5' |
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| 8101 | 3' | -61 | NC_001978.2 | + | 40315 | 0.66 | 0.375272 |
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Target: 5'- aGCCCGcuGUGCGCCcgucUCAGCG-CCUa- -3' miRNA: 3'- gCGGGC--CGUGCGGc---AGUCGCaGGAac -5' |
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| 8101 | 3' | -61 | NC_001978.2 | + | 38577 | 0.66 | 0.375272 |
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Target: 5'- gCGCCgGGCACGUCG---GCGUUCg-- -3' miRNA: 3'- -GCGGgCCGUGCGGCaguCGCAGGaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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