miRNA: 3'- cCGCGA----ACGGGUaCCGGCauUGCUag -5'
miRNA: 3'- -CCGCGa-ACGGgUaCCGGCauUGCUAG- -5'
miRNA: 3'- -CCGCG------AACGG-GUACCGGCaUUGCUag -5'
miRNA: 3'- -CCGCGAACGGGUACCGGCAUUGCUAG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8102 | 3' | -54.9 | NC_001978.2 | + | 22119 | 0.72 | 0.302445 |
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Target: 5'- uGCGCUucgcUGCCCA-GGUCGgcGCGAa- -3' miRNA: 3'- cCGCGA----ACGGGUaCCGGCauUGCUag -5' |
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| 8102 | 3' | -54.9 | NC_001978.2 | + | 19869 | 0.72 | 0.294799 |
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Target: 5'- uGGCGCcgUGCCgGgGGCCGacGCGAUUc -3' miRNA: 3'- -CCGCGa-ACGGgUaCCGGCauUGCUAG- -5' |
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| 8102 | 3' | -54.9 | NC_001978.2 | + | 32128 | 0.73 | 0.252076 |
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Target: 5'- aGGCGCgucagcUUGCCgCAUGGCUG-AACGAg- -3' miRNA: 3'- -CCGCG------AACGG-GUACCGGCaUUGCUag -5' |
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| 8102 | 3' | -54.9 | NC_001978.2 | + | 18047 | 1.13 | 0.000392 |
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Target: 5'- aGGCGCUUGCCCAUGGCCGUAACGAUCg -3' miRNA: 3'- -CCGCGAACGGGUACCGGCAUUGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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