Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 811 | 5' | -45.9 | NC_000866.4 | + | 149652 | 0.67 | 0.999934 |
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Target: 5'- -aACUUGGCguugAUGGU-AAGAUGUUUa -3' miRNA: 3'- gaUGAGCCGaa--UGCCGuUUCUAUAAA- -5' |
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| 811 | 5' | -45.9 | NC_000866.4 | + | 23809 | 0.67 | 0.999921 |
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Target: 5'- -aGCUgGGCUUucCGGCAaacaucacuaaauccAAGAUGUUUu -3' miRNA: 3'- gaUGAgCCGAAu-GCCGU---------------UUCUAUAAA- -5' |
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| 811 | 5' | -45.9 | NC_000866.4 | + | 65084 | 0.67 | 0.999884 |
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Target: 5'- cCUACUUGGUacaaauacUACGGUAAGGcgAUUUa -3' miRNA: 3'- -GAUGAGCCGa-------AUGCCGUUUCuaUAAA- -5' |
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| 811 | 5' | -45.9 | NC_000866.4 | + | 89416 | 0.68 | 0.999674 |
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Target: 5'- -gAUUCGGCUcauggaaauuauUugGGCAAGGAa---- -3' miRNA: 3'- gaUGAGCCGA------------AugCCGUUUCUauaaa -5' |
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| 811 | 5' | -45.9 | NC_000866.4 | + | 91081 | 0.71 | 0.993948 |
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Target: 5'- -aGCUaauGCUUGCGGUAGAGAUAa-- -3' miRNA: 3'- gaUGAgc-CGAAUGCCGUUUCUAUaaa -5' |
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| 811 | 5' | -45.9 | NC_000866.4 | + | 70938 | 1.06 | 0.041341 |
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Target: 5'- gCUACUCGGCUUACGGCAAAGAUAUUUc -3' miRNA: 3'- -GAUGAGCCGAAUGCCGUUUCUAUAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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