Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8110 | 3' | -53.8 | NC_001978.2 | + | 36152 | 0.66 | 0.728261 |
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Target: 5'- cGCGC-GCCcagGUGUCGGCGGguGAGUgGc -3' miRNA: 3'- -CGCGaCGG---UACAGCCGUUgcUUCAgC- -5' |
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| 8110 | 3' | -53.8 | NC_001978.2 | + | 28885 | 0.66 | 0.728261 |
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Target: 5'- aGCGCUGCCAacaCGcGCAuuuCGAuugccugaaAGUCGa -3' miRNA: 3'- -CGCGACGGUacaGC-CGUu--GCU---------UCAGC- -5' |
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| 8110 | 3' | -53.8 | NC_001978.2 | + | 12259 | 0.66 | 0.739098 |
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Target: 5'- uGCGCgGCUgacgacGUCGGCAGCGGA--CGg -3' miRNA: 3'- -CGCGaCGGua----CAGCCGUUGCUUcaGC- -5' |
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| 8110 | 3' | -53.8 | NC_001978.2 | + | 26606 | 0.66 | 0.739098 |
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Target: 5'- aUGCUGaCCuUGUCGGCuacauGCGucggcuuGUCGg -3' miRNA: 3'- cGCGAC-GGuACAGCCGu----UGCuu-----CAGC- -5' |
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| 8110 | 3' | -53.8 | NC_001978.2 | + | 21989 | 0.66 | 0.739098 |
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Target: 5'- uCGCUGCCAUcUUGGCuugccACGggG-Ca -3' miRNA: 3'- cGCGACGGUAcAGCCGu----UGCuuCaGc -5' |
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| 8110 | 3' | -53.8 | NC_001978.2 | + | 25723 | 0.66 | 0.739098 |
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Target: 5'- cGCGCucacUGCCGUaucgGUCGGCuauGACGccGUUGc -3' miRNA: 3'- -CGCG----ACGGUA----CAGCCG---UUGCuuCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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