miRNA: 3'- -UGgUGUAGCUUG-------CUAG--GCCGUAUCGG- -5'
miRNA: 3'- ugGUGUAGCUUGCUag----GCCGU-AUCGG- -5'
miRNA: 3'- -UGGUGUAGCUUGCUAGGCCGUAUCGG- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8111 | 3' | -52.3 | NC_001978.2 | + | 25485 | 0.72 | 0.438239 |
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Target: 5'- gGCgACGUCGAGCccgaaccGGUCggCGGCGUAGUCg -3' miRNA: 3'- -UGgUGUAGCUUG-------CUAG--GCCGUAUCGG- -5' |
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| 8111 | 3' | -52.3 | NC_001978.2 | + | 18501 | 0.76 | 0.267212 |
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Target: 5'- -gCACGUCGAGCGAagggaaCGGCAgUGGCCu -3' miRNA: 3'- ugGUGUAGCUUGCUag----GCCGU-AUCGG- -5' |
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| 8111 | 3' | -52.3 | NC_001978.2 | + | 22294 | 1.13 | 0.000777 |
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Target: 5'- gACCACAUCGAACGAUCCGGCAUAGCCg -3' miRNA: 3'- -UGGUGUAGCUUGCUAGGCCGUAUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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