Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8116 | 3' | -53.6 | NC_001978.2 | + | 33719 | 0.66 | 0.773989 |
Target: 5'- aCCggCUGAAGCGUCgu-CGuCUUGCCg -3' miRNA: 3'- -GGaaGGCUUCGCAGaacGC-GAGUGGg -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 25034 | 0.66 | 0.773989 |
Target: 5'- cCCaUUCgGcAAGCGUCUUcGCGCccUUGCUCa -3' miRNA: 3'- -GG-AAGgC-UUCGCAGAA-CGCG--AGUGGG- -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 34123 | 0.66 | 0.773989 |
Target: 5'- cCCUUCCGgcGUGcCcgggugUGCGUcCGCCg -3' miRNA: 3'- -GGAAGGCuuCGCaGa-----ACGCGaGUGGg -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 18060 | 0.66 | 0.773989 |
Target: 5'- cCCUUC---AGCGaCaagGCGCUUGCCCa -3' miRNA: 3'- -GGAAGgcuUCGCaGaa-CGCGAGUGGG- -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 20667 | 0.66 | 0.773989 |
Target: 5'- --cUUCGu--CGUCUUGCGCUcCGCCg -3' miRNA: 3'- ggaAGGCuucGCAGAACGCGA-GUGGg -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 31824 | 0.66 | 0.753141 |
Target: 5'- cCCggaagCCGGAcGCGUCgagUGCGUcagucCAUCCg -3' miRNA: 3'- -GGaa---GGCUU-CGCAGa--ACGCGa----GUGGG- -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 24343 | 0.66 | 0.746781 |
Target: 5'- uCCgcCCGggGCGUg-UGUcuguucagccguucuGUUCGCCCg -3' miRNA: 3'- -GGaaGGCuuCGCAgaACG---------------CGAGUGGG- -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 13154 | 0.66 | 0.742517 |
Target: 5'- gCCguaCCGGcAGCGUUcaGCGuCUCGCCg -3' miRNA: 3'- -GGaa-GGCU-UCGCAGaaCGC-GAGUGGg -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 19287 | 0.66 | 0.742517 |
Target: 5'- cCCUUCguuguCGuuGUGgugCUU-CGCUCACCCu -3' miRNA: 3'- -GGAAG-----GCuuCGCa--GAAcGCGAGUGGG- -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 15610 | 0.66 | 0.731777 |
Target: 5'- gCCUUCCGcaacAGCGaCUUcguacGCGCUgggACCCg -3' miRNA: 3'- -GGAAGGCu---UCGCaGAA-----CGCGAg--UGGG- -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 32436 | 0.66 | 0.731777 |
Target: 5'- gCCUuaCGA--CGUUcUGuCGCUCGCCCa -3' miRNA: 3'- -GGAagGCUucGCAGaAC-GCGAGUGGG- -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 41155 | 0.66 | 0.731777 |
Target: 5'- gCCaUgCGAAGCGgcgUGCuGCuaUCGCCCg -3' miRNA: 3'- -GGaAgGCUUCGCagaACG-CG--AGUGGG- -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 17531 | 0.66 | 0.720933 |
Target: 5'- ---cCCGAcGCGcacgCUUGCGCagauacacgccgUCGCCCu -3' miRNA: 3'- ggaaGGCUuCGCa---GAACGCG------------AGUGGG- -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 27211 | 0.66 | 0.720933 |
Target: 5'- ---aCCGGGcGaCGUCcgaUGCGCUCGCCg -3' miRNA: 3'- ggaaGGCUU-C-GCAGa--ACGCGAGUGGg -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 30315 | 0.66 | 0.719843 |
Target: 5'- aCCUUCacggcguuuucguCGGccccGGCGUUgccgUUGCGCUCuuCCCg -3' miRNA: 3'- -GGAAG-------------GCU----UCGCAG----AACGCGAGu-GGG- -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 21227 | 0.67 | 0.708898 |
Target: 5'- aCCUUCgCGAAGCGcuuaccggccuugUCgaccuggGCGCgaaUCACCUu -3' miRNA: 3'- -GGAAG-GCUUCGC-------------AGaa-----CGCG---AGUGGG- -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 26309 | 0.67 | 0.698981 |
Target: 5'- ----gCGcGGCG-CUUGUGCUCGCCg -3' miRNA: 3'- ggaagGCuUCGCaGAACGCGAGUGGg -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 9742 | 0.67 | 0.695662 |
Target: 5'- aUCUUCCGGagcccgucaAGCGUCUUGUcuagggcguccuucGCgaccggucCGCCCu -3' miRNA: 3'- -GGAAGGCU---------UCGCAGAACG--------------CGa-------GUGGG- -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 21033 | 0.68 | 0.647615 |
Target: 5'- gCCgUCCGucAGCGUCUccaguuccuucgugGCGCccucaaucgccuUCACCCg -3' miRNA: 3'- -GGaAGGCu-UCGCAGAa-------------CGCG------------AGUGGG- -5' |
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8116 | 3' | -53.6 | NC_001978.2 | + | 16275 | 0.68 | 0.643118 |
Target: 5'- aCCgcugCCGA--CGUUgggGCGCUCGCCa -3' miRNA: 3'- -GGaa--GGCUucGCAGaa-CGCGAGUGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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