Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 812 | 5' | -48.5 | NC_000866.4 | + | 21081 | 0.67 | 0.998839 |
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Target: 5'- cGAUAUUGCaGAAUCuGCcGGUCUUCCa -3' miRNA: 3'- -CUAUGAUGaCUUGGcUGuCUAGGAGG- -5' |
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| 812 | 5' | -48.5 | NC_000866.4 | + | 85744 | 0.68 | 0.997301 |
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Target: 5'- gGAUugcCUACUGAauauGCCGACAGAauagcuaaauuaaCUCCa -3' miRNA: 3'- -CUAu--GAUGACU----UGGCUGUCUag-----------GAGG- -5' |
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| 812 | 5' | -48.5 | NC_000866.4 | + | 128604 | 0.68 | 0.99711 |
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Target: 5'- uAUGCUagaGCUcaGGACCGugGGAcCCUCUu -3' miRNA: 3'- cUAUGA---UGA--CUUGGCugUCUaGGAGG- -5' |
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| 812 | 5' | -48.5 | NC_000866.4 | + | 111000 | 0.69 | 0.994179 |
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Target: 5'- --aACUACUuuaauagucuuuuGACCGGCAGGUCCUUUu -3' miRNA: 3'- cuaUGAUGAc------------UUGGCUGUCUAGGAGG- -5' |
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| 812 | 5' | -48.5 | NC_000866.4 | + | 50212 | 0.69 | 0.991542 |
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Target: 5'- uGAUGauucacCUGAACCGAUAGAaauUCgUCCa -3' miRNA: 3'- -CUAUgau---GACUUGGCUGUCU---AGgAGG- -5' |
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| 812 | 5' | -48.5 | NC_000866.4 | + | 20877 | 0.71 | 0.977282 |
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Target: 5'- --gGCUguuGCUGGaagACCGGCAGAUUCUgCa -3' miRNA: 3'- cuaUGA---UGACU---UGGCUGUCUAGGAgG- -5' |
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| 812 | 5' | -48.5 | NC_000866.4 | + | 119187 | 0.74 | 0.907435 |
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Target: 5'- uGAUACUGCUGAAuCUGugGG-UCCUUUa -3' miRNA: 3'- -CUAUGAUGACUU-GGCugUCuAGGAGG- -5' |
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| 812 | 5' | -48.5 | NC_000866.4 | + | 80003 | 1.13 | 0.010462 |
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Target: 5'- uGAUACUACUGAACCGACAGAUCCUCCu -3' miRNA: 3'- -CUAUGAUGACUUGGCUGUCUAGGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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