Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8126 | 5' | -56 | NC_001978.2 | + | 20019 | 0.66 | 0.644301 |
Target: 5'- gCCGggugcuCGAAGGUCGugC-CGaCCGg- -3' miRNA: 3'- gGGCau----GCUUCCAGCugGuGC-GGCaa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 11607 | 0.66 | 0.644301 |
Target: 5'- aCCGUGCGGgcAGcGUCGcCCACGauGUa -3' miRNA: 3'- gGGCAUGCU--UC-CAGCuGGUGCggCAa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 13291 | 0.66 | 0.644301 |
Target: 5'- uUCCG-GCGGcaAGGUCGGCCGuCGUgGUc -3' miRNA: 3'- -GGGCaUGCU--UCCAGCUGGU-GCGgCAa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 20518 | 0.66 | 0.622182 |
Target: 5'- gCCGUccuUGAucAGGUCGGgCACGCCu-- -3' miRNA: 3'- gGGCAu--GCU--UCCAGCUgGUGCGGcaa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 12507 | 0.66 | 0.589093 |
Target: 5'- cUCCGUcgGCGuucccGGUCGGCuuCACGCUGUa -3' miRNA: 3'- -GGGCA--UGCuu---CCAGCUG--GUGCGGCAa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 36883 | 0.66 | 0.589093 |
Target: 5'- aCCCGguu-GAGGUCGcuGCCGCuGCCGg- -3' miRNA: 3'- -GGGCaugcUUCCAGC--UGGUG-CGGCaa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 38584 | 0.67 | 0.582508 |
Target: 5'- cCCCGUcgcgcucgacuucgcGCuGAAGGUCGGCCGCcUUGUc -3' miRNA: 3'- -GGGCA---------------UG-CUUCCAGCUGGUGcGGCAa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 31577 | 0.67 | 0.578125 |
Target: 5'- -aCGUGCGAAGGcguugaggaCGAagACGCCGUUa -3' miRNA: 3'- ggGCAUGCUUCCa--------GCUggUGCGGCAA- -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 6747 | 0.67 | 0.567201 |
Target: 5'- gCCGgucACGuuGGUUGGCUucGCGCCGg- -3' miRNA: 3'- gGGCa--UGCuuCCAGCUGG--UGCGGCaa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 17602 | 0.67 | 0.556331 |
Target: 5'- -aCGUugGcGGcUCGACCACGCCu-- -3' miRNA: 3'- ggGCAugCuUCcAGCUGGUGCGGcaa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 20371 | 0.67 | 0.542292 |
Target: 5'- cCCCGgaagccgaagcgGCGAAGGcCGAagucgagUCGCGCCGg- -3' miRNA: 3'- -GGGCa-----------UGCUUCCaGCU-------GGUGCGGCaa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 11734 | 0.67 | 0.539069 |
Target: 5'- gCCGUACuGAgugaagcggcuuaccGGGUCGuCCGgCGCCGg- -3' miRNA: 3'- gGGCAUG-CU---------------UCCAGCuGGU-GCGGCaa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 5802 | 0.68 | 0.513533 |
Target: 5'- gCCGcACGAAGGUCGuuCACGaCGa- -3' miRNA: 3'- gGGCaUGCUUCCAGCugGUGCgGCaa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 28698 | 0.68 | 0.507226 |
Target: 5'- gCCCGUGCGGaucgugguuguucgcGaaccGGUCGGCgUACGUCGUUa -3' miRNA: 3'- -GGGCAUGCU---------------U----CCAGCUG-GUGCGGCAA- -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 18729 | 0.68 | 0.501994 |
Target: 5'- gCCGUACGAcgucggaaugagcGGGUCGAUUcggaGCGCUGc- -3' miRNA: 3'- gGGCAUGCU-------------UCCAGCUGG----UGCGGCaa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 35692 | 0.7 | 0.413431 |
Target: 5'- cCCCGUACGGAgccggagacGGacgacaCGGCCACGCUGc- -3' miRNA: 3'- -GGGCAUGCUU---------CCa-----GCUGGUGCGGCaa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 21484 | 0.7 | 0.413431 |
Target: 5'- uCCCGguCGggGGcUCGACUcCGCCGa- -3' miRNA: 3'- -GGGCauGCuuCC-AGCUGGuGCGGCaa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 31124 | 0.7 | 0.376879 |
Target: 5'- aCCCGggGCGGAGc-UGACCACGCaCGUUc -3' miRNA: 3'- -GGGCa-UGCUUCcaGCUGGUGCG-GCAA- -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 25733 | 0.71 | 0.359438 |
Target: 5'- gCCGUAUc--GGUCGGCUaugACGCCGUUg -3' miRNA: 3'- gGGCAUGcuuCCAGCUGG---UGCGGCAA- -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 22270 | 0.71 | 0.326277 |
Target: 5'- gCCGUACGu--GUcCGACCACGCCa-- -3' miRNA: 3'- gGGCAUGCuucCA-GCUGGUGCGGcaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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