Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8156 | 3' | -58.4 | NC_001978.2 | + | 18364 | 0.66 | 0.439384 |
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Target: 5'- gGCGCgUGcGUAGuUGCCGCCCGgcuugcugUGGUCGc -3' miRNA: 3'- -UGCG-ACcCGUC-ACGGCGGGC--------AUUAGU- -5' |
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| 8156 | 3' | -58.4 | NC_001978.2 | + | 14399 | 0.66 | 0.429644 |
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Target: 5'- cGCGCUGGGCacGGUuuaCGUCCGUuggcuUCAu -3' miRNA: 3'- -UGCGACCCG--UCAcg-GCGGGCAuu---AGU- -5' |
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| 8156 | 3' | -58.4 | NC_001978.2 | + | 9111 | 0.66 | 0.420032 |
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Target: 5'- aGCGcCUGGGUGaaGCCGCCgGUGAa-- -3' miRNA: 3'- -UGC-GACCCGUcaCGGCGGgCAUUagu -5' |
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| 8156 | 3' | -58.4 | NC_001978.2 | + | 16661 | 0.67 | 0.389259 |
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Target: 5'- -gGCUGGGCAGgaaaccggcgcgcuUGCCGCUaugcGGUCAa -3' miRNA: 3'- ugCGACCCGUC--------------ACGGCGGgca-UUAGU- -5' |
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| 8156 | 3' | -58.4 | NC_001978.2 | + | 13921 | 0.67 | 0.365205 |
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Target: 5'- uCGCUGGGCuugaaGgCGCCCGgauacagGGUCAg -3' miRNA: 3'- uGCGACCCGuca--CgGCGGGCa------UUAGU- -5' |
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| 8156 | 3' | -58.4 | NC_001978.2 | + | 40185 | 0.69 | 0.284611 |
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Target: 5'- cGCGCuucucgaUGGGCGuucccUGCCcccuGCCCGUAGUCGa -3' miRNA: 3'- -UGCG-------ACCCGUc----ACGG----CGGGCAUUAGU- -5' |
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| 8156 | 3' | -58.4 | NC_001978.2 | + | 22837 | 0.71 | 0.214116 |
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Target: 5'- cGCGCUGGGCuucauUGaCUGCCUGUAcgCGg -3' miRNA: 3'- -UGCGACCCGuc---AC-GGCGGGCAUuaGU- -5' |
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| 8156 | 3' | -58.4 | NC_001978.2 | + | 2970 | 0.75 | 0.104017 |
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Target: 5'- aGCGCUGGGgAGUGCCcccGCCCGaagAGUgCAg -3' miRNA: 3'- -UGCGACCCgUCACGG---CGGGCa--UUA-GU- -5' |
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| 8156 | 3' | -58.4 | NC_001978.2 | + | 1853 | 1.08 | 0.000342 |
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Target: 5'- gACGCUGGGCAGUGCCGCCCGUAAUCAg -3' miRNA: 3'- -UGCGACCCGUCACGGCGGGCAUUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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