Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8166 | 3' | -57.5 | NC_001978.2 | + | 15553 | 0.67 | 0.499966 |
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Target: 5'- cGuAGuCGGGcGGCa-GGGGCGACGUCg -3' miRNA: 3'- aC-UC-GCCCuUCGgcCCUUGCUGCAGg -5' |
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| 8166 | 3' | -57.5 | NC_001978.2 | + | 36464 | 0.67 | 0.499966 |
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Target: 5'- -cGGCGugacGAAGCUGGGcGCGugGcCCg -3' miRNA: 3'- acUCGCc---CUUCGGCCCuUGCugCaGG- -5' |
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| 8166 | 3' | -57.5 | NC_001978.2 | + | 13206 | 0.67 | 0.499966 |
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Target: 5'- gGGGCGGcuuGCUGuGGAccACGACGgCCg -3' miRNA: 3'- aCUCGCCcuuCGGC-CCU--UGCUGCaGG- -5' |
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| 8166 | 3' | -57.5 | NC_001978.2 | + | 5372 | 0.66 | 0.520771 |
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Target: 5'- cGGGuCGGGcgaccGCCGGGAuuguCGGCGagacggCCg -3' miRNA: 3'- aCUC-GCCCuu---CGGCCCUu---GCUGCa-----GG- -5' |
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| 8166 | 3' | -57.5 | NC_001978.2 | + | 32227 | 0.66 | 0.566548 |
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Target: 5'- cUGAGCGGcagcgcgccauucgcGAAGCgcucaacgucgacCGGGucACGACGcCCa -3' miRNA: 3'- -ACUCGCC---------------CUUCG-------------GCCCu-UGCUGCaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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