Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8171 | 3' | -64.5 | NC_001978.2 | + | 22880 | 0.68 | 0.176916 |
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Target: 5'- cGCGGGGACggCGGCGUugUGGcCGugCCa -3' miRNA: 3'- cCGUCCCUG--GCCGCG--GCCuGCugGGc -5' |
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| 8171 | 3' | -64.5 | NC_001978.2 | + | 27308 | 0.67 | 0.230013 |
|
Target: 5'- cGGCAgcgucgagcacgcccGGGAagaaaCCGGCGagcgcaUCGGACGucGCCCGg -3' miRNA: 3'- -CCGU---------------CCCU-----GGCCGC------GGCCUGC--UGGGC- -5' |
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| 8171 | 3' | -64.5 | NC_001978.2 | + | 28009 | 0.72 | 0.100222 |
|
Target: 5'- cGGCAuuGGGACCGGCcucaaGCCGcucagugcguucuacGuCGACCCGu -3' miRNA: 3'- -CCGU--CCCUGGCCG-----CGGC---------------CuGCUGGGC- -5' |
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| 8171 | 3' | -64.5 | NC_001978.2 | + | 34596 | 0.66 | 0.263837 |
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Target: 5'- cGGCAGGG-UCGGa-CCGG-CGAgCCa -3' miRNA: 3'- -CCGUCCCuGGCCgcGGCCuGCUgGGc -5' |
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| 8171 | 3' | -64.5 | NC_001978.2 | + | 34711 | 0.66 | 0.245239 |
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Target: 5'- uGCAacGGGGCucaguCGGCGUCGGcuCGaACCCGg -3' miRNA: 3'- cCGU--CCCUG-----GCCGCGGCCu-GC-UGGGC- -5' |
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| 8171 | 3' | -64.5 | NC_001978.2 | + | 36964 | 0.68 | 0.17243 |
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Target: 5'- uGGCAGGGugccgACCGGCaG-CGGcaGCGACCUc -3' miRNA: 3'- -CCGUCCC-----UGGCCG-CgGCC--UGCUGGGc -5' |
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| 8171 | 3' | -64.5 | NC_001978.2 | + | 40280 | 0.68 | 0.186202 |
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Target: 5'- gGGCAGcGGgaagggucgGCCGGUcccCCGGuCGGCCCa -3' miRNA: 3'- -CCGUC-CC---------UGGCCGc--GGCCuGCUGGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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