Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8197 | 5' | -54 | NC_001978.2 | + | 23068 | 0.66 | 0.660403 |
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Target: 5'- -aGCACUCAUGGCGaAGCgUUCGaucuGGg -3' miRNA: 3'- uaCGUGAGUACUGCcUCG-AAGCg---UCa -5' |
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| 8197 | 5' | -54 | NC_001978.2 | + | 21584 | 0.66 | 0.648862 |
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Target: 5'- -aGCACguaccGACGGGGCUUCGUc-- -3' miRNA: 3'- uaCGUGagua-CUGCCUCGAAGCGuca -5' |
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| 8197 | 5' | -54 | NC_001978.2 | + | 34253 | 0.66 | 0.625742 |
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Target: 5'- cAUGCGC-CGUcGGCGGAGCUUgccggagccaCGCuGUa -3' miRNA: 3'- -UACGUGaGUA-CUGCCUCGAA----------GCGuCA- -5' |
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| 8197 | 5' | -54 | NC_001978.2 | + | 8729 | 0.67 | 0.556866 |
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Target: 5'- cGUGCGCg---GcAUGGAGCUUCGCGa- -3' miRNA: 3'- -UACGUGaguaC-UGCCUCGAAGCGUca -5' |
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| 8197 | 5' | -54 | NC_001978.2 | + | 24305 | 0.68 | 0.534337 |
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Target: 5'- aAUGCGCuUCGUGcCGGGGCUugUCGUcGUu -3' miRNA: 3'- -UACGUG-AGUACuGCCUCGA--AGCGuCA- -5' |
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| 8197 | 5' | -54 | NC_001978.2 | + | 39576 | 0.68 | 0.511039 |
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Target: 5'- -cGCGCuucgaagUCAUGACGGcGCcacacUCGCAGUa -3' miRNA: 3'- uaCGUG-------AGUACUGCCuCGa----AGCGUCA- -5' |
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| 8197 | 5' | -54 | NC_001978.2 | + | 35353 | 0.69 | 0.458462 |
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Target: 5'- cAUGCGgUCcagAUGACGGAGCcgCGCAc- -3' miRNA: 3'- -UACGUgAG---UACUGCCUCGaaGCGUca -5' |
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| 8197 | 5' | -54 | NC_001978.2 | + | 18906 | 0.7 | 0.437841 |
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Target: 5'- -cGCGCUCuucGUGAcCGGGGCUUCaGCAu- -3' miRNA: 3'- uaCGUGAG---UACU-GCCUCGAAG-CGUca -5' |
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| 8197 | 5' | -54 | NC_001978.2 | + | 32829 | 0.7 | 0.398254 |
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Target: 5'- cGUGCGCUUcUGACGGAGCUgaGCcGa -3' miRNA: 3'- -UACGUGAGuACUGCCUCGAagCGuCa -5' |
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| 8197 | 5' | -54 | NC_001978.2 | + | 26374 | 1.06 | 0.001141 |
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Target: 5'- gAUGCACUCAUGACGGAGCUUCGCAGUg -3' miRNA: 3'- -UACGUGAGUACUGCCUCGAAGCGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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