Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
82 | 5' | -48.1 | AC_000005.1 | + | 8362 | 0.66 | 0.909634 |
Target: 5'- aGCGGgGGaagcgGUAUGUCGGCcuGACg-- -3' miRNA: 3'- -CGCCgCCa----CAUACGGUUGuuUUGaau -5' |
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82 | 5' | -48.1 | AC_000005.1 | + | 15254 | 0.66 | 0.916635 |
Target: 5'- aUGGCGGUGguugGUcuacGCCGGCGAcGCa-- -3' miRNA: 3'- cGCCGCCACa---UA----CGGUUGUUuUGaau -5' |
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82 | 5' | -48.1 | AC_000005.1 | + | 18225 | 0.68 | 0.842167 |
Target: 5'- gGCGGCGGUGauaGUGUCGcUAAGAUa-- -3' miRNA: 3'- -CGCCGCCACa--UACGGUuGUUUUGaau -5' |
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82 | 5' | -48.1 | AC_000005.1 | + | 32946 | 0.7 | 0.757783 |
Target: 5'- aUGGCGGUGgagggcGCCcuuACAGAGCUUu -3' miRNA: 3'- cGCCGCCACaua---CGGu--UGUUUUGAAu -5' |
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82 | 5' | -48.1 | AC_000005.1 | + | 18231 | 1.1 | 0.002202 |
Target: 5'- cGCGGCGGUGUAUGCCAACAAAACUUAu -3' miRNA: 3'- -CGCCGCCACAUACGGUUGUUUUGAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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