Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8206 | 5' | -55.2 | NC_001978.2 | + | 39511 | 0.66 | 0.640881 |
Target: 5'- uGGCGcCGUCAUGacuucGAAGCGCGggGAAGa -3' miRNA: 3'- -CUGCcGCAGUGU-----CUUCGCGCgaCUUC- -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 29093 | 0.66 | 0.640881 |
Target: 5'- gGGCGuCGUCGCGuuggcagcguGAAGCGCGCggcacGggGc -3' miRNA: 3'- -CUGCcGCAGUGU----------CUUCGCGCGa----CuuC- -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 8106 | 0.66 | 0.634098 |
Target: 5'- cACGGCcuuguucaauuccuuGUCGCGGAGcGCGCGCa---- -3' miRNA: 3'- cUGCCG---------------CAGUGUCUU-CGCGCGacuuc -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 30643 | 0.66 | 0.618272 |
Target: 5'- cGGCGGaCGUUGCGgcgcuuGAAGaCGUGCUGAc- -3' miRNA: 3'- -CUGCC-GCAGUGU------CUUC-GCGCGACUuc -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 15413 | 0.66 | 0.618272 |
Target: 5'- -cCGGCGUCGuuGgcGCGCGCa---- -3' miRNA: 3'- cuGCCGCAGUguCuuCGCGCGacuuc -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 18597 | 0.66 | 0.618272 |
Target: 5'- cGGCaGCGUUACGGAuaucAGCGCgucgcauucGCUGAc- -3' miRNA: 3'- -CUGcCGCAGUGUCU----UCGCG---------CGACUuc -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 40215 | 0.66 | 0.616012 |
Target: 5'- aGCGGCGUagcgagaCACccgGGAAGCcuguuagGCGCUGAGa -3' miRNA: 3'- cUGCCGCA-------GUG---UCUUCG-------CGCGACUUc -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 19957 | 0.66 | 0.606981 |
Target: 5'- aACGGCGUUcCGG-AGCGCG-UGAAu -3' miRNA: 3'- cUGCCGCAGuGUCuUCGCGCgACUUc -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 22946 | 0.66 | 0.595713 |
Target: 5'- --gGGCGUCACGauGAGCGCGa-GAAGc -3' miRNA: 3'- cugCCGCAGUGUc-UUCGCGCgaCUUC- -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 19535 | 0.66 | 0.595713 |
Target: 5'- cACGGCGUCACcGAucGUGCGCc---- -3' miRNA: 3'- cUGCCGCAGUGuCUu-CGCGCGacuuc -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 16276 | 0.67 | 0.573284 |
Target: 5'- cACGGCGUU-CGGGucGGCaaGCUGggGg -3' miRNA: 3'- cUGCCGCAGuGUCU--UCGcgCGACuuC- -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 17420 | 0.67 | 0.540044 |
Target: 5'- cGGCGGCaaCACGaacacGAacGGCaGCGCUGAGGg -3' miRNA: 3'- -CUGCCGcaGUGU-----CU--UCG-CGCGACUUC- -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 24575 | 0.67 | 0.53347 |
Target: 5'- cGGCGGCGUCAUGGucggcauaugugucGGCGUGCUu--- -3' miRNA: 3'- -CUGCCGCAGUGUCu-------------UCGCGCGAcuuc -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 25561 | 0.67 | 0.529104 |
Target: 5'- cGACGGCGUgACccguggcgcccAGggGCGUGacCUGAGc -3' miRNA: 3'- -CUGCCGCAgUG-----------UCuuCGCGC--GACUUc -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 26055 | 0.69 | 0.465429 |
Target: 5'- uGACGGCGUCguGCAGu--CG-GCUGggGa -3' miRNA: 3'- -CUGCCGCAG--UGUCuucGCgCGACuuC- -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 36416 | 0.69 | 0.465429 |
Target: 5'- uGCGGCGuUCAUcauGgcGCugaGCGCUGAAGg -3' miRNA: 3'- cUGCCGC-AGUGu--CuuCG---CGCGACUUC- -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 33774 | 0.69 | 0.435111 |
Target: 5'- --aGGCGUUcCGcGAAGCGUGCUGGGc -3' miRNA: 3'- cugCCGCAGuGU-CUUCGCGCGACUUc -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 20471 | 0.7 | 0.405967 |
Target: 5'- gGACGGCGUCAagcucuucucuCAG-GGCGUcgaccugggGCUGAAGc -3' miRNA: 3'- -CUGCCGCAGU-----------GUCuUCGCG---------CGACUUC- -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 22054 | 0.71 | 0.351512 |
Target: 5'- aAUGGCGccuUCGCGGucGCGCGCUGc-- -3' miRNA: 3'- cUGCCGC---AGUGUCuuCGCGCGACuuc -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 744 | 0.72 | 0.310243 |
Target: 5'- --aGGCG-Cu--GAAGCGCGCUGAGGc -3' miRNA: 3'- cugCCGCaGuguCUUCGCGCGACUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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