Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 8217 | 5' | -59.3 | NC_001978.2 | + | 9664 | 0.71 | 0.214957 |
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Target: 5'- uCGGCG-CCgUUCAGCCC-CAUggacuuguacGCCGCg -3' miRNA: 3'- -GCCGCuGGgGAGUUGGGaGUA----------CGGCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 29017 | 0.71 | 0.214957 |
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Target: 5'- cCGGCG-CCCUUgAacACCUUCcgGUCGCg -3' miRNA: 3'- -GCCGCuGGGGAgU--UGGGAGuaCGGCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 29850 | 0.72 | 0.188388 |
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Target: 5'- gGGCGACCCUUCcggguGCCgaCUCcUGCCGa -3' miRNA: 3'- gCCGCUGGGGAGu----UGG--GAGuACGGCg -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 2024 | 0.74 | 0.121914 |
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Target: 5'- aCGGuCGACCaCCUUGACCCggaCGUGUgGCg -3' miRNA: 3'- -GCC-GCUGG-GGAGUUGGGa--GUACGgCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 1906 | 0.74 | 0.132447 |
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Target: 5'- cCGGaccCGACCCCgacgGACCCUU-UGCCGCa -3' miRNA: 3'- -GCC---GCUGGGGag--UUGGGAGuACGGCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 20304 | 0.74 | 0.13993 |
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Target: 5'- uCGGCGGCUuccuucacggCCUCAACCgUCucgGCCGUc -3' miRNA: 3'- -GCCGCUGG----------GGAGUUGGgAGua-CGGCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 14870 | 0.76 | 0.094813 |
|
Target: 5'- uCGGCG-UCCCg-AACCUUCAUGCCGUu -3' miRNA: 3'- -GCCGCuGGGGagUUGGGAGUACGGCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 32480 | 0.85 | 0.019953 |
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Target: 5'- aGGCGuaaGCCCCUCAGCCCagUCGUGCgGCu -3' miRNA: 3'- gCCGC---UGGGGAGUUGGG--AGUACGgCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 37765 | 1.11 | 0.000199 |
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Target: 5'- aCGGCGACCCCUCAACCCUCAUGCCGCu -3' miRNA: 3'- -GCCGCUGGGGAGUUGGGAGUACGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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