Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8217 | 5' | -59.3 | NC_001978.2 | + | 30365 | 0.66 | 0.45428 |
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Target: 5'- gGGCGGCUUCguaCAcgucGCCCUCG-GCgGCu -3' miRNA: 3'- gCCGCUGGGGa--GU----UGGGAGUaCGgCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 20463 | 0.66 | 0.434953 |
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Target: 5'- uCGGcCGACCggcgcgacucgaCUUCGGCCUUC--GCCGCu -3' miRNA: 3'- -GCC-GCUGG------------GGAGUUGGGAGuaCGGCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 40343 | 0.66 | 0.434953 |
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Target: 5'- cCGGcCGACCCUUCccgcuGCCC-CAggaaGcCCGCu -3' miRNA: 3'- -GCC-GCUGGGGAGu----UGGGaGUa---C-GGCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 9937 | 0.66 | 0.434953 |
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Target: 5'- cCGcGCGugaACgCCUUGAUCUUCGUuGCCGCg -3' miRNA: 3'- -GC-CGC---UGgGGAGUUGGGAGUA-CGGCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 16002 | 0.66 | 0.406879 |
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Target: 5'- uCGGCG-CCCgUCGcGCCC--GUGUCGCc -3' miRNA: 3'- -GCCGCuGGGgAGU-UGGGagUACGGCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 33001 | 0.67 | 0.379969 |
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Target: 5'- --aCGGCUUCUCAAgCUcCGUGCCGCg -3' miRNA: 3'- gccGCUGGGGAGUUgGGaGUACGGCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 29206 | 0.68 | 0.349287 |
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Target: 5'- aGGCgccGACCCgUgAcggacacagugacaaGCCC-CGUGCCGCg -3' miRNA: 3'- gCCG---CUGGGgAgU---------------UGGGaGUACGGCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 39528 | 0.68 | 0.327459 |
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Target: 5'- uGGCGGacagaauggcuugcCCCCgggaaAGCCCU--UGCCGCg -3' miRNA: 3'- gCCGCU--------------GGGGag---UUGGGAguACGGCG- -5' |
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| 8217 | 5' | -59.3 | NC_001978.2 | + | 32480 | 0.85 | 0.019953 |
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Target: 5'- aGGCGuaaGCCCCUCAGCCCagUCGUGCgGCu -3' miRNA: 3'- gCCGC---UGGGGAGUUGGG--AGUACGgCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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