Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8222 | 3' | -60.4 | NC_001978.2 | + | 39900 | 1 | 0.001065 |
Target: 5'- cGCUGACGCUCCGGCAGCAA-GGGCGGa -3' miRNA: 3'- -CGACUGCGAGGCCGUCGUUcCCCGCC- -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 13301 | 0.75 | 0.096971 |
Target: 5'- aGCgUGAgCGUUCCGGCGGCAAGGucGGCc- -3' miRNA: 3'- -CG-ACU-GCGAGGCCGUCGUUCC--CCGcc -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 15634 | 0.73 | 0.124475 |
Target: 5'- gGCUcGACgGUUCCGGCGGCuucgcuGGGGCu- -3' miRNA: 3'- -CGA-CUG-CGAGGCCGUCGuu----CCCCGcc -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 17336 | 0.71 | 0.167401 |
Target: 5'- --cGGCGCUCaGGUguucuucGGCAacGGGGGCGGc -3' miRNA: 3'- cgaCUGCGAGgCCG-------UCGU--UCCCCGCC- -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 15556 | 0.71 | 0.172404 |
Target: 5'- --cGGCGUagUCgGGCGGC-AGGGGCGa -3' miRNA: 3'- cgaCUGCG--AGgCCGUCGuUCCCCGCc -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 4606 | 0.71 | 0.191738 |
Target: 5'- uGCUcGACGg-UgGGCAGCGAcGGGCGGa -3' miRNA: 3'- -CGA-CUGCgaGgCCGUCGUUcCCCGCC- -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 12534 | 0.7 | 0.212947 |
Target: 5'- cGCUGuACGaCUCCGGCAcCAAucagacauGGGuGCGGc -3' miRNA: 3'- -CGAC-UGC-GAGGCCGUcGUU--------CCC-CGCC- -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 39417 | 0.7 | 0.212947 |
Target: 5'- aGCUucaGGCGUggUUGGaCGGCAGGGGGCGcGg -3' miRNA: 3'- -CGA---CUGCGa-GGCC-GUCGUUCCCCGC-C- -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 12133 | 0.7 | 0.215176 |
Target: 5'- uGCgGuCgGCUCCGGCacgguccuuaucgggGGCAcggcuGGGGGCGGc -3' miRNA: 3'- -CGaCuG-CGAGGCCG---------------UCGU-----UCCCCGCC- -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 13178 | 0.69 | 0.261418 |
Target: 5'- -gUGGUGCUCCGcGaCGGCGAacccgacuGGGGCGGc -3' miRNA: 3'- cgACUGCGAGGC-C-GUCGUU--------CCCCGCC- -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 13257 | 0.68 | 0.274862 |
Target: 5'- cGCUGaACGCgUCCgGGUGGCAcAGcuauuacgccGGGCGGg -3' miRNA: 3'- -CGAC-UGCG-AGG-CCGUCGU-UC----------CCCGCC- -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 20669 | 0.68 | 0.281789 |
Target: 5'- uGCcGACGaCgacgUUGaGCGGCAGGGGGCGc -3' miRNA: 3'- -CGaCUGC-Ga---GGC-CGUCGUUCCCCGCc -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 7806 | 0.68 | 0.303398 |
Target: 5'- cGUUGAgGCUCCGGCgauucAGUucacGGGCGa -3' miRNA: 3'- -CGACUgCGAGGCCG-----UCGuuc-CCCGCc -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 41371 | 0.67 | 0.3185 |
Target: 5'- --aGACGgcaauggaCUUCGGCaAGCGuccguucugaGGGGGCGGg -3' miRNA: 3'- cgaCUGC--------GAGGCCG-UCGU----------UCCCCGCC- -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 34204 | 0.67 | 0.332568 |
Target: 5'- gGC--ACGCUCCGGCggacgcacacccgGGCAcgccggaAGGGGCa- -3' miRNA: 3'- -CGacUGCGAGGCCG-------------UCGU-------UCCCCGcc -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 13092 | 0.67 | 0.342198 |
Target: 5'- cGCUGaACGCUgCCGGUacGGCGAcGGuaGGa -3' miRNA: 3'- -CGAC-UGCGA-GGCCG--UCGUUcCCcgCC- -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 12264 | 0.67 | 0.342198 |
Target: 5'- gGCUGACGacgUCGGCAGCGGacGGCu- -3' miRNA: 3'- -CGACUGCga-GGCCGUCGUUccCCGcc -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 33006 | 0.67 | 0.347907 |
Target: 5'- cCUGAacggcuucucaaGCUCCGuGCcgcgaauGGCAGGGGGCa- -3' miRNA: 3'- cGACUg-----------CGAGGC-CG-------UCGUUCCCCGcc -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 1328 | 0.67 | 0.350375 |
Target: 5'- cGCUGACGCgaCGGUucccAGUGAGGGuuacCGGg -3' miRNA: 3'- -CGACUGCGagGCCG----UCGUUCCCc---GCC- -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 1757 | 0.67 | 0.350375 |
Target: 5'- cGCUGAacguCGCacaCGGCGGCAAGcuucGGGCu- -3' miRNA: 3'- -CGACU----GCGag-GCCGUCGUUC----CCCGcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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